Imaging Experimental Intraabdominal Abscesses with 99mTc-PEG Liposomes and 99mTc-HYNIC IgG

Overview

Journal Ann Surg

Specialty General Surgery

Date 1999 Apr 15

PMID 10203089

Citations 10

Authors

E T Dams

M M Reijnen

W J Oyen

O C Boerman

P Laverman

G Storm

J W van der Meer

F H Corstens

H van Goor

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the accuracy of technetium-99m-labeled polyethylene glycol-coated liposomes (99mTc-PEG liposomes) and technetium-99m-labeled nonspecific human immunoglobulin G (99mTc-HYNIC IgG) for the scintigraphic detection of experimental intraabdominal abscesses in comparison with that of a standard agent, gallium-67 citrate.

Background: Scintigraphic imaging techniques can be very useful for the rapid and accurate localization of intraabdominal abscesses. Two newly developed radiolabeled agents, 99mTc-PEG liposomes and 99mTc-HYNIC IgG, have shown to be excellent agents for imaging experimental focal infection, but have not yet been studied in the detection of abdominal abscesses.

Methods: Intraabdominal abscesses were induced in 42 rats using the cecal ligation and puncture technique. Seven days later, randomized groups of rats received 99mTc-PEG liposomes, 99mTc-HYNIC IgG, or 67Ga citrate intravenously. The rats were imaged up to 24 hours after the injection. The biodistribution of the radiolabel was determined by counting dissected tissues ex vivo. Macroscopic intraabdominal abnormalities and focal uptake on the images were independently scored on a semiquantitative scale.

Results: 99mTc-PEG liposomes provided the earliest scintigraphic visualization of the abscess (as soon as 2 hours after the injection vs. 4 hours for the other two agents). Liposomes, IgG, and gallium all showed similarly high absolute uptake in the abscess. Focal uptake of liposomes and gallium correlated best with the extent of the macroscopic abnormalities.

Conclusions: 99mTc-PEG liposomes and 99mTc-HYNIC IgG performed at least as well as the standard agent, 67Ga citrate, in the detection of experimental intraabdominal abscesses, with obvious advantages such as lower radiation exposure and more favorable physical properties. Of the two technetium agents, the liposomes seemed to be superior, providing the earliest diagnostic image and the best correlation with the inflammatory abnormalities. In addition, the preferential localization of radiolabeled PEG liposomes holds promise for targeted delivery of liposome-encapsulated drugs.

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References

Wichterman K, Baue A, Chaudry I . Sepsis and septic shock--a review of laboratory models and a proposal. J Surg Res. 1980; 29(2):189-201. DOI: 10.1016/0022-4804(80)90037-2. View

Laverman P, Dams E, Oyen W, Storm G, Koenders E, Prevost R . A novel method to label liposomes with 99mTc by the hydrazino nicotinyl derivative. J Nucl Med. 1999; 40(1):192-7. View

Bohnen J, Boulanger M, Meakins J, McLean A . Prognosis in generalized peritonitis. Relation to cause and risk factors. Arch Surg. 1983; 118(3):285-90. DOI: 10.1001/archsurg.1983.01390030017003. View

Seabold J, Wilson D, Lieberman L, Boyd C . Unsuspected extra-abdominal sites of infection: scintigraphic detection with indium-111-labeled leukocytes. Radiology. 1984; 151(1):213-7. DOI: 10.1148/radiology.151.1.6701317. View

Scholmerich J, Schmidt E, Schumichen C, Billmann P, Schmidt H, Gerok W . Scintigraphic assessment of bowel involvement and disease activity in Crohn's disease using technetium 99m-hexamethyl propylene amine oxine as leukocyte label. Gastroenterology. 1988; 95(5):1287-93. DOI: 10.1016/0016-5085(88)90363-0. View

Rotstein O, Kao J . Prevention of intra-abdominal abscesses by fibrinolysis using recombinant tissue plasminogen activator. J Infect Dis. 1988; 158(4):766-72. DOI: 10.1093/infdis/158.4.766. View

Gagliardi P, Hoffer P, Rosenfield A . Correlative imaging in abdominal infection: an algorithmic approach using nuclear medicine, ultrasound, and computed tomography. Semin Nucl Med. 1988; 18(4):320-34. DOI: 10.1016/s0001-2998(88)80041-2. View

Morrell E, Tompkins R, Fischman A, Wilkinson R, Burke J, Rubin R . Autoradiographic method for quantitation of radiolabeled proteins in tissues using indium-111. J Nucl Med. 1989; 30(9):1538-45. View

Oyen W, Claessens R, van Horn J, van der Meer J, Corstens F . Scintigraphic detection of bone and joint infections with indium-111-labeled nonspecific polyclonal human immunoglobulin G. J Nucl Med. 1990; 31(4):403-12. View

10.

Vipond M, Whawell S, Thompson J, Dudley H . Peritoneal fibrinolytic activity and intra-abdominal adhesions. Lancet. 1990; 335(8698):1120-2. DOI: 10.1016/0140-6736(90)91125-t. View

11.

Abrams M, Juweid M, tenKate C, Schwartz D, HAUSER M, Gaul F . Technetium-99m-human polyclonal IgG radiolabeled via the hydrazino nicotinamide derivative for imaging focal sites of infection in rats. J Nucl Med. 1990; 31(12):2022-8. View

12.

Arndt J, van der Sluys Veer A, Blok D, Griffioen G, Verspaget H, Pena A . A prospective comparison of 99mTc-labeled polyclonal human immunoglobulin and 111In granulocytes for localization of inflammatory bowel disease. Acta Radiol. 1992; 33(2):140-4. View

13.

Phillips W, RUDOLPH A, Goins B, Timmons J, Klipper R, Blumhardt R . A simple method for producing a technetium-99m-labeled liposome which is stable in vivo. Int J Rad Appl Instrum B. 1992; 19(5):539-47. DOI: 10.1016/0883-2897(92)90149-s. View

14.

Lokerse A, ten Kate M, Mouton J, Woodle M, Storm G . Liposomes with prolonged blood circulation and selective localization in Klebsiella pneumoniae-infected lung tissue. J Infect Dis. 1993; 168(1):164-71. DOI: 10.1093/infdis/168.1.164. View

15.

Fry D . Noninvasive imaging tests in the diagnosis and treatment of intra-abdominal abscesses in the postoperative patient. Surg Clin North Am. 1994; 74(3):693-709. View

16.

Palestro C . The current role of gallium imaging in infection. Semin Nucl Med. 1994; 24(2):128-41. DOI: 10.1016/s0001-2998(05)80227-2. View

17.

van Goor H, de Graaf J, Kooi K, Sluiter W, Bom V, van der Meer J . Effect of recombinant tissue plasminogen activator on intra-abdominal abscess formation in rats with generalized peritonitis. J Am Coll Surg. 1994; 179(4):407-11. View

18.

Lantto E . Investigation of suspected intra-abdominal sepsis: the contribution of nuclear medicine. Scand J Gastroenterol Suppl. 1994; 203:11-4. DOI: 10.3109/00365529409091389. View

19.

McLauchlan G, Anderson I, Grant I, Fearon K . Outcome of patients with abdominal sepsis treated in an intensive care unit. Br J Surg. 1995; 82(4):524-9. DOI: 10.1002/bjs.1800820429. View

20.

Oyen W, Boerman O, van der Laken C, Claessens R, van der Meer J, Corstens F . The uptake mechanisms of inflammation- and infection-localizing agents. Eur J Nucl Med. 1996; 23(4):459-65. DOI: 10.1007/BF01247377. View