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Yunrong Gao

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Articles 14
Citations 314
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Recent Articles
1.
Gao Y, Raghavan A, Garcia S, Deng B, Hurtado de Mendoza D, Liang B
J Virol . 2024 Jul; 98(8):e0104624. PMID: 39016557
The respiratory syncytial virus (RSV) M2-1 protein is a transcriptional antitermination factor crucial for efficiently synthesizing multiple full-length viral mRNAs. During RSV infection, M2-1 exists in a complex with mRNA...
2.
Cao D, Gao Y, Chen Z, Gooneratne I, Roesler C, Mera C, et al.
Nature . 2023 Dec; 625(7995):611-617. PMID: 38123676
The respiratory syncytial virus (RSV) polymerase is a multifunctional RNA-dependent RNA polymerase composed of the large (L) protein and the phosphoprotein (P). It transcribes the RNA genome into ten viral...
3.
Ahn H, Calderon B, Fan X, Gao Y, Horgan N, Jiang N, et al.
J Med Virol . 2023 Oct; 95(10):e29163. PMID: 37842796
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) enters the host cell by binding to angiotensin-converting enzyme 2 (ACE2). While evolutionarily conserved, ACE2 receptors differ across various species and differential interactions...
4.
Gao Y, Raghavan A, Deng B, Lee J, Liang B
Viruses . 2023 Feb; 15(2). PMID: 36851557
The nucleocapsids (NCs) of the respiratory syncytial virus (RSV) can display multiple morphologies in vivo, including spherical, asymmetric, and filamentous conformations. Obtaining homogeneous ring-like oligomers in vitro is significant since...
5.
Gao Y, Ogilvie C, Raghavan A, Von Hoffmann C, Liang B
J Vis Exp . 2021 Aug; (173). PMID: 34398139
The use of an authentic RNA template is critical to advance the fundamental knowledge of viral RNA synthesis that can guide both mechanistic discovery and assay development in virology. The...
6.
Cao D, Gao Y, Liang B
Viruses . 2021 Jun; 13(5). PMID: 34063087
RNA synthesis in respiratory syncytial virus (RSV), a negative-sense (-) nonsegmented RNA virus, consists of viral gene transcription and genome replication. Gene transcription includes the positive-sense (+) viral mRNA synthesis,...
7.
Gao Y, Cao D, Pawnikar S, Akhter S, Miao Y, Liang B
STAR Protoc . 2021 Mar; 2(1):100315. PMID: 33659898
Here, we describe a generic protocol for monitoring protein-RNA interaction using a cleavable GFP fusion of a recombinant RNA-binding protein. We detail each expression and purification step, including high salt...
8.
Cao D, Gao Y, Roesler C, Rice S, DCunha P, Zhuang L, et al.
J Virol . 2020 Oct; 95(1). PMID: 33028717
Respiratory syncytial virus (RSV) is a nonsegmented negative-sense (NNS) RNA virus and shares a similar RNA synthesis strategy with other members of NNS RNA viruses, such as measles, rabies virus,...
9.
Gao Y, Cao D, Pawnikar S, John K, Ahn H, Hill S, et al.
Structure . 2020 Jul; 28(9):979-990.e4. PMID: 32697936
The M2-1 protein of human respiratory syncytial virus (HRSV) is a transcription anti-terminator that regulates the processivity of the HRSV RNA-dependent RNA polymerase (RdRP). Here, we report a crystal structure...
10.
Cao D, Gao Y, Roesler C, Rice S, DCunha P, Zhuang L, et al.
Nat Commun . 2020 Jan; 11(1):368. PMID: 31953395
The respiratory syncytial virus (RSV) RNA polymerase, constituted of a 250 kDa large (L) protein and tetrameric phosphoprotein (P), catalyzes three distinct enzymatic activities - nucleotide polymerization, cap addition, and...