Yoshitaka Shirasaki
Overview
Explore the profile of Yoshitaka Shirasaki including associated specialties, affiliations and a list of published articles.
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Articles
30
Citations
598
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Recent Articles
21.
Kamatani T, Fukunaga K, Miyata K, Shirasaki Y, Tanaka J, Baba R, et al.
Sci Rep
. 2017 Dec;
7(1):16831.
PMID: 29203784
For single-cell experiments, it is important to accurately count the number of viable cells in a nanoliter well. We used a deep learning-based convolutional neural network (CNN) on a large...
22.
Liu T, Yamaguchi Y, Shirasaki Y, Shikada K, Yamagishi M, Hoshino K, et al.
Cell Rep
. 2014 Aug;
8(4):974-82.
PMID: 25127135
Inflammasome-mediated caspase-1 activation is involved in cell death and the secretion of the proinflammatory cytokine interleukin-1β (IL-1β). Although the dynamics of caspase-1 activation, IL-1β secretion, and cell death have been...
23.
Shirasaki Y, Yamagishi M, Suzuki N, Izawa K, Nakahara A, Mizuno J, et al.
Sci Rep
. 2014 Apr;
4:4736.
PMID: 24751898
Protein secretion, a key intercellular event for transducing cellular signals, is thought to be strictly regulated. However, secretion dynamics at the single-cell level have not yet been clarified because intercellular...
24.
Shirasaki Y, Yamagishi M, Shimura N, Hijikata A, Ohara O
IUBMB Life
. 2013 Jan;
65(1):28-34.
PMID: 23281035
The immune system is a very complex and dynamic cellular system, and its intricacies are considered akin to those of human society. Disturbance of homeostasis of the immune system results...
25.
Yamagishi M, Shirasaki Y, Funatsu T
Methods Mol Biol
. 2012 Oct;
950:153-67.
PMID: 23086875
Some mRNAs localize to specific regions within eukaryotic cells to express their functions. The movement and localization of mRNA molecules provides valuable information about how they concentrate to particular regions....
26.
Sugino H, Arakawa T, Nara Y, Shirasaki Y, Ozaki K, Shoji S, et al.
Lab Chip
. 2010 Aug;
10(19):2559-65.
PMID: 20689871
Microfluidic systems have significant implications in the field of cell separation since they could provide platforms with inexpensive, disposable and sterile structures. Here, we present a novel strategy to integrate...
27.
Aoyama C, Saeki A, Noguchi M, Shirasaki Y, Shoji S, Funatsu T, et al.
Anal Chem
. 2010 Jan;
82(4):1420-6.
PMID: 20092311
In this study, we show for the first time that the separation efficiency of a pillar array column under pressure-driven liquid chromatography (LC) conditions can be improved using a separation...
28.
Yamagishi M, Shirasaki Y, Funatsu T
Biochem Biophys Res Commun
. 2009 Oct;
390(3):750-4.
PMID: 19835844
beta-actin mRNA localizes to the leading edge of a living chicken embryo fibroblast. Recently we proposed that the mRNA maintains its localization at the leading edge by utilizing the heterogeneity...
29.
Sugino H, Ozaki K, Shirasaki Y, Arakawa T, Shoji S, Funatsu T
Lab Chip
. 2009 Apr;
9(9):1254-60.
PMID: 19370245
The microfluidic platform is an important tool for diagnosis and biomedical studies because it enables us to handle precious cells and infectious materials safely. We have developed an on-chip microfluidic...
30.
Yamagishi M, Ishihama Y, Shirasaki Y, Kurama H, Funatsu T
Exp Cell Res
. 2009 Feb;
315(7):1142-7.
PMID: 19245805
Beta-actin mRNA labeled with an MS2-EGFP fusion protein was expressed in chicken embryo fibroblasts and its localization and movement were analyzed by single-molecule imaging. Most beta-Actin mRNAs localized to the...