Tomoya Kozakai
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Explore the profile of Tomoya Kozakai including associated specialties, affiliations and a list of published articles.
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6
Citations
27
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Recent Articles
1.
Kozakai T, Nakajima A, Miyazawa K, Sasaki Y, Odamaki T, Katoh T, et al.
iScience
. 2024 Nov;
27(11):111080.
PMID: 39502284
is a prevalent bacterial taxon in the human gut that comprises over 10 (sub)species. Previous studies suggest that these species use evolutionarily distinct strategies for symbiosis with their hosts. However,...
2.
Altaib H, Kozakai T, Badr Y, Nakao H, El-Nouby M, Yanase E, et al.
Microb Cell Fact
. 2022 Mar;
21(1):33.
PMID: 35255900
Background: Bifidobacteria are gram-positive, probiotic, and generally regarded as safe bacteria. Techniques such as transformation, gene knockout, and heterologous gene expression have been established for Bifidobacterium, indicating that this bacterium...
3.
Altaib H, Ozaki Y, Kozakai T, Sakaguchi K, Nomura I, Suzuki T
Microbiol Resour Announc
. 2022 Jan;
11(1):e0088421.
PMID: 35023783
A series of -Escherichia coli shuttle vectors (pKO403--Cm, pKO403--Sp, pKO403--p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the α fragment, overhung...
4.
Kozakai T, Shimofusa Y, Nomura I, Suzuki T
Biosci Microbiota Food Health
. 2021 May;
40(2):115-122.
PMID: 33996368
A reporter assay system is an essential tool for investigating gene expression mechanisms. In the case of bifidobacteria, several convenient and sensitive reporter systems have been developed. Here, we developed...
5.
Kozakai T, Izumi A, Horigome A, Odamaki T, Xiao J, Nomura I, et al.
J Bacteriol
. 2020 Jan;
202(7).
PMID: 31964699
Bacterial promoters consist of core sequence motifs termed -35 and -10 boxes. The consensus motifs are TTGACA and TATAAT, respectively, which were identified from leading investigations on However, the consensus...
6.
Altaib H, Ozaki Y, Kozakai T, Badr Y, Nomura I, Suzuki T
Microbiol Resour Announc
. 2019 Oct;
8(41).
PMID: 31601653
A series of new entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two...