Seiichi Toki
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Explore the profile of Seiichi Toki including associated specialties, affiliations and a list of published articles.
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107
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3212
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Recent Articles
1.
Nagamura R, Kujirai T, Kato J, Shuto Y, Kusakizako T, Hirano H, et al.
Nat Commun
. 2024 Dec;
15(1):10744.
PMID: 39737984
The CRISPR-associated endonuclease Cas9 derived from prokaryotes is used as a genome editing, which targets specific genomic loci by single guide RNAs (sgRNAs). The eukaryotes, the target of genome editing,...
2.
Endo M, Negishi K, Toki S
Methods Mol Biol
. 2024 Nov;
2869:101-111.
PMID: 39499471
Base editors, CRISPR/Cas-based precise genome editing tools, enable base conversion at a target site without inducing DNA double-strand breaks. The genome editing targetable range is restricted by the requirement for...
3.
Nishizawa-Yokoi A, Toki S
Methods Mol Biol
. 2024 Nov;
2869:91-100.
PMID: 39499470
Gene targeting (GT) is a precise genome editing tool to achieve desired modification of a target gene, e.g., introduction of point mutations, knock-in of a reporter gene, or swapping of...
4.
Sukegawa S, Toki S, Saika H
Methods Mol Biol
. 2024 Nov;
2869:75-90.
PMID: 39499469
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR-Cas) is an adaptive prokaryote immune system against foreign DNA/RNA that is now applied widely to genome editing. A...
5.
Ishibashi K, Sukegawa S, Endo M, Hara N, Nureki O, Saika H, et al.
Front Plant Sci
. 2024 Sep;
15:1454554.
PMID: 39323536
Because virus vectors can spread systemically autonomously, they are powerful vehicles with which to deliver genome-editing tools into plant cells. Indeed, a vector based on a positive-strand RNA virus, potato...
6.
Yoshida T, Ishikawa M, Toki S, Ishibashi K
Plant Cell Physiol
. 2024 Aug;
65(11):1743-1750.
PMID: 39215594
Conventional plant gene editing requires laborious tissue-culture-mediated transformation, which restricts the range of applicable plant species. In this study, we developed a heritable and tissue-culture-free gene editing method in Nicotiana...
7.
Nishizawa-Yokoi A, Toki S
Plant Biotechnol (Tokyo)
. 2024 Mar;
40(4):255-262.
PMID: 38434112
Transposons are mobile genetic elements that can move to a different position within a genome or between genomes. They have long been used as a tool for genetic engineering, including...
8.
Kishi-Kaboshi M, Nishizawa-Yokoi A, Mitsuhara I, Toki S, Sasaki K
Plant Biotechnol (Tokyo)
. 2024 Jan;
40(2):157-165.
PMID: 38250294
is one of the most popular ornamental plants in the world. However, as is a segmental hexaploid, self-incompatible, and has a sizable heterologous genome, it is difficult to modify its...
9.
Nishiguchi M, Futamura N, Endo M, Mikami M, Toki S, Katahata S, et al.
Sci Rep
. 2023 Jul;
13(1):11779.
PMID: 37479866
Sugi (Cryptomeria japonica D. Don) is an economically important coniferous tree in Japan. However, abundant sugi pollen grains are dispersed and transported by the wind each spring and cause a...
10.
Nakajima I, Kawahigashi H, Nishitani C, Azuma A, Haji T, Toki S, et al.
PLoS One
. 2023 Jun;
18(6):e0286698.
PMID: 37289779
Transposition of transposable elements affect expression levels, splicing and epigenetic status, and function of genes located in, or near, the inserted/excised locus. For example, in grape, presence of the Gret1...