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S Yoast

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Articles 7
Citations 47
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Recent Articles
1.
Babe L, Yoast S, Dreyer M, Schmidt B
Biotechnol Appl Biochem . 1998 May; 27(2):117-24. PMID: 9569606
Human granzyme K, a serine protease found in secretory granules of cytotoxic T-lymphocytes, was produced in its catalytically active form by recombinant technology using Bacillus subtilis as host. The enzyme...
2.
Przetak M, Yoast S, Schmidt B
FEBS Lett . 1995 May; 364(3):268-71. PMID: 7758581
A serine protease gene was cloned from cDNA prepared from tissue isolated from human ascites. The gene codes for a protein of 264 amino acids, identified as human granzyme 3...
3.
Mendez M, Abderrahim H, Noguchi M, David N, Hardy M, Green L, et al.
Genomics . 1995 Mar; 26(2):294-307. PMID: 7601456
With the goal of creating a strain of mice capable of producing human antibodies, we are cloning and reconstructing the human immunoglobulin germline repertoire in yeast artificial chromosomes (YACs). We...
4.
Moon K, Yoast S, Palombella A, Mainzer S, Schmidt B
Biochem Biophys Res Commun . 1994 Jun; 201(3):1167-74. PMID: 8024558
Twelve tyrosines, ten glutamates, and five histidines were individually substituted for phenylalanine, glutamine, and asparagine, respectively, in the beta-galactosidase from Lactobacillus delbrückii subsp. bulgaricus. Only Y509, E464, H351, and H534...
5.
Yoast S, ADAMS R, Mainzer S, Moon K, Palombella A, Schmidt B
Appl Environ Microbiol . 1994 Apr; 60(4):1221-6. PMID: 16349230
A method is described for generating and screening variants of the beta-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus sensitive to several environmental stresses, with potential application in the food industry. Chemical...
6.
ADAMS R, Yoast S, Mainzer S, Moon K, Palombella A, Estell D, et al.
J Biol Chem . 1994 Feb; 269(8):5666-72. PMID: 8119903
Methoxylamine mutagenesis of the beta-galactosidase gene from Lactobacillus delbrückii subsp. bulgaricus was used to generate cold-sensitive variants. Two variants, P429S and L317F, were characterized kinetically in order to determine the...
7.
Triman K, Becker E, Dammel C, Katz J, Mori H, Douthwaite S, et al.
J Mol Biol . 1989 Oct; 209(4):645-53. PMID: 2531227
Temperature-sensitive mutants have been isolated following hydroxylamine mutagenesis of a plasmid containing Escherichia coli rRNA genes carrying selectable markers for spectinomycin resistance (U1192 in 16 S rRNA) and erythromycin resistance...