» Authors » S E Halford

S E Halford

Explore the profile of S E Halford including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 93
Citations 1241
Followers 0
Related Specialties
Top 10 Co-Authors
Published In
Affiliations
Soon will be listed here.
Recent Articles
11.
Gormley N, Bath A, Halford S
J Biol Chem . 2000 Mar; 275(10):6928-36. PMID: 10702254
Type II restriction enzymes generally recognize continuous sequences of 4-8 consecutive base pairs on DNA, but some recognize discontinuous sites where the specified sequence is interrupted by a defined length...
12.
Baldwin G, Gormley N, Halford S
Met Ions Biol Syst . 2000 Feb; 37:345-64. PMID: 10693139
No abstract available.
13.
Bilcock D, DANIELS L, Bath A, Halford S
J Biol Chem . 1999 Dec; 274(51):36379-86. PMID: 10593932
Type II restriction endonucleases usually recognize 4-6-base pair (bp) sites on DNA and cleave each site in a separate reaction. A few type II endonucleases have 8-bp recognition sites, but...
14.
Thomas M, Brady R, Halford S, Sessions R, Baldwin G
Nucleic Acids Res . 1999 Aug; 27(17):3438-45. PMID: 10446231
Following random mutagenesis of the Eco RV endonuclease, a high proportion of the null mutants carry substitutions at Gln69. Such mutants display reduced rates for the DNA cleavage step in...
15.
Embleton M, Williams S, Watson M, Halford S
J Mol Biol . 1999 Jun; 289(4):785-97. PMID: 10369761
The synapsis of DNA sites is a prerequisite for the reactions of many proteins that act at specific DNA sequences. The requirement for synapsis was investigated by analysing the reactions...
16.
Stanford N, Halford S, Baldwin G
J Mol Biol . 1999 May; 288(1):105-16. PMID: 10329129
To characterise the pH dependence of phosphodiester hydrolysis by the EcoRV endonuclease in the presence of Mn2+, single turnover reactions on a 12 bp DNA substrate were examined by stopped-flow...
17.
Baldwin G, Sessions R, Erskine S, Halford S
J Mol Biol . 1999 May; 288(1):87-103. PMID: 10329128
The roles of divalent metal ions in DNA cleavage by the EcoRV endonuclease were studied by using Co2+ or Mn2+ as substitutes for the natural cofactor Mg2+. In steady-state experiments...
18.
Mizuuchi K, Nobbs T, Halford S, Adzuma K, Qin J
Biochemistry . 1999 Apr; 38(14):4640-8. PMID: 10194386
A new method was developed for tracking the stereochemical path of enzymatic cleavage of DNA. DNA with a phosphorothioate of known chirality at the scissile bond is cleaved by the...
19.
Bilcock D, Halford S
Mol Microbiol . 1999 Mar; 31(4):1243-54. PMID: 10096090
In contrast to many type II restriction enzymes, dimeric proteins that cleave DNA at individual recognition sites 4-6 bp long, the SfiI endonuclease is a tetrameric protein that binds to...
20.
Wentzell L, Halford S
J Mol Biol . 1998 Aug; 281(3):433-44. PMID: 9698559
The SfiI endonuclease has to interact with two copies of its recognition sequence before it can cleave DNA. To demonstrate that the reaction of SfiI on a DNA with two...