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Robert Craigie

Explore the profile of Robert Craigie including associated specialties, affiliations and a list of published articles. Areas
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Articles 51
Citations 1481
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Recent Articles
1.
Li M, Li Z, Chen X, Cui Y, Engelman A, Craigie R
Viruses . 2024 Jul; 16(7). PMID: 39066328
Retroviral integration is mediated by intasome nucleoprotein complexes wherein a pair of viral DNA ends are bridged together by a multimer of integrase (IN). Atomic-resolution structures of HIV-1 intasomes provide...
2.
Li M, Yang R, Chen X, Wang H, Ghirlando R, Dimitriadis E, et al.
J Mol Biol . 2024 Apr; 436(10):168557. PMID: 38582148
Retroviral DNA integration is mediated by nucleoprotein complexes (intasomes) in which a pair of viral DNA ends are bridged by a multimer of integrase (IN). Most of the high-resolution structures...
3.
Mahajan P, Smith S, Li M, Craigie R, Hughes S, Zhao X, et al.
ACS Infect Dis . 2024 Feb; 10(3):917-927. PMID: 38346249
HIV-1 integrase (IN) is an important molecular target for the development of anti-AIDS drugs. A recently FDA-approved second-generation integrase strand transfer inhibitor (INSTI) cabotegravir (CAB, 2021) is being marketed for...
4.
Jing T, Shan Z, Dinh T, Biswas A, Jang S, Greenwood J, et al.
bioRxiv . 2024 Feb; PMID: 38328132
Integrase (IN) performs dual essential roles during HIV-1 replication. During ingress, IN functions within an oligomeric "intasome" assembly to catalyze viral DNA integration into host chromatin. During late stages of...
5.
Li M, Oliveira Passos D, Shan Z, Smith S, Sun Q, Biswas A, et al.
Sci Adv . 2023 Jul; 9(29):eadg5953. PMID: 37478179
HIV-1 infection depends on the integration of viral DNA into host chromatin. Integration is mediated by the viral enzyme integrase and is blocked by integrase strand transfer inhibitors (INSTIs), first-line...
6.
Sapp N, Burge N, Cox K, Prakash P, Balasubramaniam M, Thapa S, et al.
J Virol . 2022 Sep; 96(18):e0101122. PMID: 36094316
HIV-1 DNA is preferentially integrated into chromosomal hot spots by the preintegration complex (PIC). To understand the mechanism, we measured the DNA integration activity of PICs-extracted from infected cells-and intasomes,...
7.
Oliveira Passos D, Li M, Craigie R, Lyumkis D
Enzymes . 2021 Dec; 50:249-300. PMID: 34861940
The retroviral protein Integrase (IN) catalyzes concerted integration of viral DNA into host chromatin to establish a permanent infection in the target cell. We learned a great deal about the...
8.
Craigie R
Hum Gene Ther . 2021 Nov; 32(21-22):1309-1310. PMID: 34780291
No abstract available.
9.
Davids B, Balasubramaniam M, Sapp N, Prakash P, Ingram S, Li M, et al.
J Virol . 2021 Jun; 95(17):e0055521. PMID: 34105995
Three prime repair exonuclease 1 (TREX1) is the most abundant 3'→5' exonuclease in mammalian cells. It has been suggested that TREX1 degrades HIV-1 DNA to enable the virus to evade...
10.
Cai M, Huang Y, Lloyd J, Craigie R, Clore G
J Biomol NMR . 2021 Feb; 75(2-3):83-87. PMID: 33538948
A simple and cost-effective protocol is presented for expression of perdeuterated, Ile/Leu/Val H/C methyl protonated proteins from 100 ml cultures in M9 ++ /DO medium induced at high (OD ~ ...