Peter van Ulsen
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Explore the profile of Peter van Ulsen including associated specialties, affiliations and a list of published articles.
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Recent Articles
11.
Phan T, Kuijl C, Huynh D, Jong W, Luirink J, van Ulsen P
Microb Cell Fact
. 2021 Sep;
20(1):176.
PMID: 34488755
Monomeric autotransporters have been used extensively to transport recombinant proteins or protein domains to the cell surface of Gram-negative bacteria amongst others for antigen display. Genetic fusion of such antigens...
12.
Steenhuis M, van Ulsen P, Martin N, Luirink J
FEMS Microbiol Lett
. 2021 May;
368(11).
PMID: 34048543
Gram-negative pathogens are a rapidly increasing threat to human health worldwide due to high rates of antibiotic resistance and the lack of development of novel antibiotics. The protective cell envelope...
13.
Steenhuis M, Ten Hagen-Jongman C, van Ulsen P, Luirink J
Antibiotics (Basel)
. 2020 Nov;
9(11).
PMID: 33202774
The structural integrity of the Gram-negative cell envelope is guarded by several stress responses, such as the σ, Cpx and Rcs systems. Here, we report on assays that monitor these...
14.
Peschke M, Le Goff M, Koningstein G, Vischer N, Abdel-Rehim A, High S, et al.
mBio
. 2019 Oct;
10(5).
PMID: 31615956
Tail-anchored membrane proteins (TAMPs) are a distinct subset of inner membrane proteins (IMPs) characterized by a single C-terminal transmembrane domain (TMD) that is responsible for both targeting and anchoring. Little...
15.
Steenhuis M, Abdallah A, de Munnik S, Kuhne S, Sterk G, van den Berg van Saparoea B, et al.
Mol Microbiol
. 2019 Apr;
112(1):81-98.
PMID: 30983025
Disarming pathogens by targeting virulence factors is a promising alternative to classic antibiotics. Many virulence factors in Gram-negative bacteria are secreted via the autotransporter (AT) pathway, also known as Type...
16.
van Ulsen P, Zinner K, Jong W, Luirink J
FEMS Microbiol Lett
. 2018 Aug;
365(18).
PMID: 30085010
The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria to export virulence and colonization factors to their cell surface or into their surroundings. They are expressed as monomeric proteins...
17.
Jong W, Schillemans M, Ten Hagen-Jongman C, Luirink J, van Ulsen P
PLoS One
. 2018 Feb;
13(2):e0191622.
PMID: 29415042
Monomeric autotransporters have been extensively used for export of recombinant proteins to the cell surface of Gram-negative bacteria. A bottleneck in the biosynthesis of such constructs is the passage of...
18.
Peschke M, Le Goff M, Koningstein G, Karyolaimos A, Gier J, van Ulsen P, et al.
J Mol Biol
. 2017 Dec;
430(3):389-403.
PMID: 29246766
Tail-anchored membrane proteins (TAMPs) are relatively simple membrane proteins characterized by a single transmembrane domain (TMD) at their C-terminus. Consequently, the hydrophobic TMD, which acts as a subcellular targeting signal,...
19.
Piet J, van Ulsen P, Rahman S, Bovenkerk S, Bentley S, van de Beek D, et al.
Infect Immun
. 2016 Jun;
84(9):2534-40.
PMID: 27324486
Two-partner secretion (TPS) systems export large TpsA proteins to the surface and extracellular milieu. In meningococci, three different TPS systems exist, and of these, TPS system 2 (TPS2) and TPS3...
20.
Baclayon M, van Ulsen P, Mouhib H, Hashemi Shabestari M, Verzijden T, Abeln S, et al.
ACS Nano
. 2016 May;
10(6):5710-9.
PMID: 27219538
The backbone of secreted autotransporter passenger proteins generally attains a stable β-helical structure. The secretion of passengers across the outer membrane was proposed to be driven by sequential folding of...