P Tomme
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Explore the profile of P Tomme including associated specialties, affiliations and a list of published articles.
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Articles
31
Citations
834
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Recent Articles
1.
Boraston A, Creagh A, Alam M, Kormos J, Tomme P, Haynes C, et al.
Biochemistry
. 2001 May;
40(21):6240-7.
PMID: 11371185
The C-terminal family 9 carbohydrate-binding module of xylanase 10A from Thermotoga maritima (CBM9-2) binds to amorphous cellulose, crystalline cellulose, and the insoluble fraction of oat spelt xylan. The association constants...
2.
Ma L, Bluyssen H, De Raeymaeker M, Laurysens V, van der Beek N, Pavliska H, et al.
J Virol Methods
. 2001 Apr;
93(1-2):181-8.
PMID: 11311357
Replication defective adenoviruses have been used as vectors in a variety of settings including gene transfer, gene manipulation, and functionality studies. A quantitative real-time PCR-based assay is described for rapid...
3.
Boraston A, Tomme P, Amandoron E, Kilburn D
Biochem J
. 2000 Sep;
350 Pt 3:933-41.
PMID: 10970811
The C-terminal module of xylanase 10A from Streptomyces lividans is a family 13 carbohydrate-binding module (CBM13). CBM13 binds mono- and oligo-saccharides with association constants of approximately 1x10(2) M(-1)-1x10(3) M(-1). It...
4.
Kormos J, Johnson P, Brun E, Tomme P, McIntosh L, Haynes C, et al.
Biochemistry
. 2000 Jul;
39(30):8844-52.
PMID: 10913296
Endoglucanase C (CenC), a beta1,4 glucanase from the soil bacterium Cellulomonas fimi, binds to amorphous cellulose via two homologous cellulose binding domains, termed CBD(N1) and CBD(N2). In this work, the...
5.
Brun E, Johnson P, Creagh A, Tomme P, Webster P, Haynes C, et al.
Biochemistry
. 2000 Mar;
39(10):2445-58.
PMID: 10704194
The 1,4-beta-glucanase CenC from Cellulomonas fimi contains two cellulose-binding domains, CBD(N1) and CBD(N2), arranged in tandem at its N-terminus. These homologous CBDs are distinct in their selectivity for binding amorphous...
6.
Tomme P, Boraston A, McLean B, Kormos J, Creagh A, Sturch K, et al.
J Chromatogr B Biomed Sci Appl
. 1998 Oct;
715(1):283-96.
PMID: 9792516
Cellulose-binding domains (CBDs) are discrete protein modules found in a large number of carbohydrolases and a few nonhydrolytic proteins. To date, almost 200 sequences can be classified in 13 different...
7.
Johnson P, Creagh A, Brun E, Joe K, Tomme P, Haynes C, et al.
Biochemistry
. 1998 Sep;
37(37):12772-81.
PMID: 9737854
The interaction of the N-terminal cellulose-binding domain, CBDN1, from Cellulomonas fimi beta-1,4-glucanase CenC with calcium was investigated using NMR spectroscopy and calorimetry. CBDN1 binds a single calcium ion with an...
8.
Creagh A, Koska J, Johnson P, Tomme P, Joshi M, McIntosh L, et al.
Biochemistry
. 1998 Apr;
37(10):3529-37.
PMID: 9521674
Differential scanning calorimetry has been used to study the thermal stability and oligosaccharide-binding thermodynamics of the N-terminal cellulose-binding domain of Cellulomonas fimi beta-1,4-glucanase CenC (CBDN1). CBDN1 has a relatively low...
9.
Johnson P, Joshi M, Tomme P, Kilburn D, McIntosh L
Biochemistry
. 1996 Nov;
35(45):14381-94.
PMID: 8916925
Multidimensional heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the tertiary structure of the 152 amino acid N-terminal cellulose-binding domain from Cellulomonas fimi 1,4-beta-glucanase CenC (CBDN1). CBDN1 was...
10.
Johnson P, Tomme P, Joshi M, McIntosh L
Biochemistry
. 1996 Nov;
35(44):13895-906.
PMID: 8909286
The N-terminal cellulose-binding domain (CBDN1) from Cellulomonas fimi beta-1,4-glucanase CenC binds amorphous but not crystalline cellulose. To investigate the structural and thermodynamic bases of cellulose binding, NMR and difference ultraviolet...