P D Siebert
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Explore the profile of P D Siebert including associated specialties, affiliations and a list of published articles.
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39
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2130
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Recent Articles
1.
Siebert P
Methods Mol Med
. 2011 Mar;
13:55-79.
PMID: 21390838
Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the...
2.
Siebert P
Methods Mol Med
. 2011 Mar;
13:29-53.
PMID: 21390837
Control of gene transcription, the process in which a gene's DNA sequence serves as a template for mRNA synthesis, plays a critical role in the multistep process that regulates gene...
3.
Siebert P
Methods Mol Med
. 2011 Feb;
26:61-85.
PMID: 21340870
Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the...
4.
Zhumabayeva B, Chenchik A, Siebert P, Herrler M
Adv Biochem Eng Biotechnol
. 2004 Apr;
86:191-213.
PMID: 15088766
The identification of differentially expressed genes enables us to understand the molecular mechanisms associated with disease, conditions of stress, drug treatments and developmental processes. Microarrays provide a powerful tool for...
5.
Shen Z, Peedikayil J, Olson G, Siebert P, Fang Y
Biotechniques
. 2002 May;
32(5):1168, 1170-2, 1174 passim.
PMID: 12019791
Transcription factor-DNA interactions have been widely studied in the regulation of gene expression. We have established an enzyme-linked immunoassay platform to quantify specific transcriptionfactor-DNA interactions. In our assay, dsDNA immobilized...
6.
Wiechen K, Diatchenko L, Agoulnik A, Scharff K, Schober H, ARLT K, et al.
Am J Pathol
. 2001 Nov;
159(5):1635-43.
PMID: 11696424
To identify novel markers differentially expressed in ovarian cancer versus normal ovary, we hybridized microarrays with cDNAs derived from normal human ovaries and advanced stage ovarian carcinomas. This analysis revealed...
7.
Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction
Zhu Y, Machleder E, Chenchik A, Li R, Siebert P
Biotechniques
. 2001 Apr;
30(4):892-7.
PMID: 11314272
Here, we describe a fast, simple method for constructing full-length cDNA libraries using SMART technology. This novel procedure uses the template-switching activity of Moloney murine leukemia virus (MMLV) reverse transcriptase...
8.
Zhumabayeva B, Chang C, McKinley J, Diatchenko L, Siebert P
Biotechniques
. 2001 Mar;
30(3):512-6, 518-20.
PMID: 11252787
Here, we describe the application of a RecA-based cloning technology to generate full-length cDNA libraries enriched for genes that are differentially expressed between tumor and normal tissue samples. First, we...
9.
Zhumabayeva B, Diatchenko L, Chenchik A, Siebert P
Biotechniques
. 2001 Feb;
30(1):158-63.
PMID: 11196307
We demonstrate here that SMART PCR-amplified cDNAs arrayed on a nylon membrane are suitable for high-throughput tissue expression profiling when starting biological materials are limited. We show that SMART cDNA...
10.
Zhumabayeva B, Chenchik A, Siebert P
Biotechniques
. 1999 Oct;
27(4):834-6, 838, 840 passim.
PMID: 10524326
We describe an improved method for rapid cloning of full-length cDNA from cDNA libraries. This approach is based on the ability of Escherichia coli RecA protein to form a stable...