Author: Minchen Chien

1.

Discovery of SARS-CoV-2 antiviral synergy between remdesivir and approved drugs in human lung cells

Nguyenla X, Wehri E, Van Dis E, Biering S, Yamashiro L, Zhu C, et al.

Sci Rep . 2022 Nov; 12(1):18506. PMID: 36323770

SARS coronavirus 2 (SARS-CoV-2) has caused an ongoing global pandemic with significant mortality and morbidity. At this time, the only FDA-approved therapeutic for COVID-19 is remdesivir, a broad-spectrum antiviral nucleoside...

2.

Combination of antiviral drugs inhibits SARS-CoV-2 polymerase and exonuclease and demonstrates COVID-19 therapeutic potential in viral cell culture

Wang X, Sacramento C, Jockusch S, Augusto Chaves O, Tao C, Fintelman-Rodrigues N, et al.

Commun Biol . 2022 Feb; 5(1):154. PMID: 35194144

SARS-CoV-2 has an exonuclease-based proofreader, which removes nucleotide inhibitors such as Remdesivir that are incorporated into the viral RNA during replication, reducing the efficacy of these drugs for treating COVID-19....

3.

Combination of Antiviral Drugs to Inhibit SARS-CoV-2 Polymerase and Exonuclease as Potential COVID-19 Therapeutics

Wang X, Sacramento C, Jockusch S, Augusto Chaves O, Tao C, Fintelman-Rodrigues N, et al.

bioRxiv . 2021 Jul; PMID: 34312622

SARS-CoV-2 has an exonuclease-based proofreader, which removes nucleotide inhibitors such as Remdesivir that are incorporated into the viral RNA during replication, reducing the efficacy of these drugs for treating COVID-19....

4.

In vitro antiviral activity of the anti-HCV drugs daclatasvir and sofosbuvir against SARS-CoV-2, the aetiological agent of COVID-19

Sacramento C, Fintelman-Rodrigues N, Temerozo J, da Silva A, da Silva Gomes Dias S, da Silva C, et al.

J Antimicrob Chemother . 2021 Apr; 76(7):1874-1885. PMID: 33880524

Background: Current approaches of drug repurposing against COVID-19 have not proven overwhelmingly successful and the SARS-CoV-2 pandemic continues to cause major global mortality. SARS-CoV-2 nsp12, its RNA polymerase, shares homology...

5.

Nucleotide analogues as inhibitors of SARS-CoV Polymerase

Ju J, Li X, Kumar S, Jockusch S, Chien M, Tao C, et al.

Pharmacol Res Perspect . 2020 Oct; 8(6):e00674. PMID: 33124786

SARS-CoV-2, a member of the coronavirus family, has caused a global public health emergency. Based on our analysis of hepatitis C virus and coronavirus replication, and the molecular structures and...

6.

Sofosbuvir terminated RNA is more resistant to SARS-CoV-2 proofreader than RNA terminated by Remdesivir

Jockusch S, Tao C, Li X, Chien M, Kumar S, Morozova I, et al.

Sci Rep . 2020 Oct; 10(1):16577. PMID: 33024223

SARS-CoV-2 is responsible for COVID-19, resulting in the largest pandemic in over a hundred years. After examining the molecular structures and activities of hepatitis C viral inhibitors and comparing hepatitis...

7.

Nucleotide Analogues as Inhibitors of SARS-CoV-2 Polymerase, a Key Drug Target for COVID-19

Chien M, Anderson T, Jockusch S, Tao C, Li X, Kumar S, et al.

J Proteome Res . 2020 Jul; 19(11):4690-4697. PMID: 32692185

SARS-CoV-2 is responsible for the current COVID-19 pandemic. On the basis of our analysis of hepatitis C virus and coronavirus replication, and the molecular structures and activities of viral inhibitors,...

8.

A library of nucleotide analogues terminate RNA synthesis catalyzed by polymerases of coronaviruses that cause SARS and COVID-19

Jockusch S, Tao C, Li X, Anderson T, Chien M, Kumar S, et al.

Antiviral Res . 2020 Jun; 180:104857. PMID: 32562705

SARS-CoV-2, a member of the coronavirus family, is responsible for the current COVID-19 worldwide pandemic. We previously demonstrated that five nucleotide analogues inhibit the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), including...

9.

Nucleotide Analogues as Inhibitors of SARS-CoV-2 Polymerase

Chien M, Anderson T, Jockusch S, Tao C, Kumar S, Li X, et al.

bioRxiv . 2020 Jun; PMID: 32511320

SARS-CoV-2, a member of the coronavirus family, is responsible for the current COVID-19 pandemic. Based on our analysis of hepatitis C virus and coronavirus replication, and the molecular structures and...

10.

Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array

Stranges P, Palla M, Kalachikov S, Nivala J, Dorwart M, Trans A, et al.

Proc Natl Acad Sci U S A . 2016 Oct; 113(44):E6749-E6756. PMID: 27729524

Scalable, high-throughput DNA sequencing is a prerequisite for precision medicine and biomedical research. Recently, we presented a nanopore-based sequencing-by-synthesis (Nanopore-SBS) approach, which used a set of nucleotides with polymer tags...