Michiel Akeroyd
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Explore the profile of Michiel Akeroyd including associated specialties, affiliations and a list of published articles.
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15
Citations
224
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Recent Articles
1.
van Schaick G, Pot S, Schouten O, den Hartog J, Akeroyd M, van der Hoeven R, et al.
Food Chem
. 2023 Apr;
421:136147.
PMID: 37087987
Protein glycation may occur naturally when reducing sugars and proteins coexist, which is often the case for industrial enzymes. The impact of post-translational modifications on enzyme performance (e.g., stability or...
2.
van Schaick G, Dominguez-Vega E, Gstottner C, van den Berg-Verleg J, Schouten O, Akeroyd M, et al.
J Proteome Res
. 2021 Sep;
20(10):4875-4885.
PMID: 34515489
The prolyl-alanyl-specific endoprotease (EndoPro) is an industrial enzyme produced in . EndoPro is mainly used for food applications but also as a protease in proteomics. In-depth characterization of this enzyme...
3.
van der Laarse S, van Gelder C, Bern M, Akeroyd M, Olsthoorn M, Heck A
FEBS J
. 2019 Dec;
287(14):2979-2997.
PMID: 31863553
Mass spectrometry-based proteomics experiments typically start with the digestion of proteins using trypsin, chosen because of its high specificity, availability, and ease of use. It has become apparent that the...
4.
Tsiatsiani L, Akeroyd M, Olsthoorn M, Heck A
Anal Chem
. 2017 Jun;
89(15):7966-7973.
PMID: 28657298
To monitor the structural integrity of therapeutic proteins, hydrogen-deuterium exchange mass spectrometry (HDX-MS) is increasingly utilized in the pharmaceutical industry. The successful outcome of HDX-MS analyses depends on the sample...
5.
Turk S, Kloosterman W, Ninaber D, Kolen K, Knutova J, Suir E, et al.
ACS Synth Biol
. 2015 Oct;
5(1):65-73.
PMID: 26511532
Nylon-6 is a bulk polymer used for many applications. It consists of the non-natural building block 6-aminocaproic acid, the linear form of caprolactam. Via a retro-synthetic approach, two synthetic pathways...
6.
Commichau F, Alzinger A, Sande R, Bretzel W, Reuss D, Dormeyer M, et al.
Metab Eng
. 2015 Mar;
29:196-207.
PMID: 25777134
Until now, pyridoxine (PN), the most commonly supplemented B6 vitamer for animals and humans, is chemically synthesized for commercial purposes. Thus, the development of a microbial fermentation process is of...
7.
van Munster J, Nitsche B, Akeroyd M, Dijkhuizen L, van der Maarel M, Ram A
PLoS One
. 2015 Jan;
10(1):e0116269.
PMID: 25629352
Background: The filamentous fungus Aspergillus niger encounters carbon starvation in nature as well as during industrial fermentations. In response, regulatory networks initiate and control autolysis and sporulation. Carbohydrate-active enzymes play...
8.
Kozak B, van Rossum H, Luttik M, Akeroyd M, Benjamin K, Wu L, et al.
mBio
. 2014 Oct;
5(5):e01696-14.
PMID: 25336454
The energetic (ATP) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. Cytosolic acetyl coenzyme A (acetyl-CoA) is a key precursor for biosynthesis...
9.
Stam H, Akeroyd M, Menke H, Jellema R, Valianpour F, Heijne W, et al.
Methods Mol Biol
. 2013 Feb;
985:391-406.
PMID: 23417814
Genomics is based on the ability to determine the transcriptome, proteome, and metabolome of a cell. These technologies only have added value when they are integrated and based on robust...
10.
Akeroyd M, Olsthoorn M, Gerritsma J, Gutker-Vermaas D, Ekkelkamp L, van Rij T, et al.
J Biotechnol
. 2012 Dec;
164(1):112-20.
PMID: 23220267
In the discovery of new enzymes genomic and cDNA expression libraries containing thousands of differential clones are generated to obtain biodiversity. These libraries need to be screened for the activity...