Mark I Donnelly
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Explore the profile of Mark I Donnelly including associated specialties, affiliations and a list of published articles.
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11
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989
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Recent Articles
1.
Eschenfeldt W, Makowska-Grzyska M, Stols L, Donnelly M, Jedrzejczak R, Joachimiak A
J Struct Funct Genomics
. 2013 Sep;
14(4):135-44.
PMID: 24057978
In the effort to produce proteins coded by diverse genomes, structural genomics projects often must express genes containing codons that are rare in the production strain. To address this problem,...
2.
Eschenfeldt W, Maltseva N, Stols L, Donnelly M, Gu M, Nocek B, et al.
J Struct Funct Genomics
. 2010 Mar;
11(1):31-9.
PMID: 20213425
High-throughput structural genomics projects seek to delineate protein structure space by determining the structure of representatives of all major protein families. Generally this is accomplished by processing numerous proteins through...
3.
Graslund S, Nordlund P, Weigelt J, Hallberg B, Bray J, Gileadi O, et al.
Nat Methods
. 2008 Feb;
5(2):135-46.
PMID: 18235434
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a...
4.
Stols L, Zhou M, Eschenfeldt W, Millard C, Abdullah J, Collart F, et al.
Protein Expr Purif
. 2007 Mar;
53(2):396-403.
PMID: 17363272
The Bacillus subtilis genes scoA and scoB encode subunits of the heteromeric enzyme ScoAB, a putative succinyl-CoA:acetoacetate coenzyme A transferase. High-throughput, ligation-independent cloning (LIC) vectors used extensively for production and...
5.
Donnelly M, Zhou M, Millard C, Clancy S, Stols L, Eschenfeldt W, et al.
Protein Expr Purif
. 2006 Feb;
47(2):446-54.
PMID: 16497515
Production of milligram quantities of numerous proteins for structural and functional studies requires an efficient purification pipeline. We found that the dual tag, his(6)-tag-maltose-binding protein (MBP), intended to facilitate purification...
6.
Eirich L, Craft D, Steinberg L, Asif A, Eschenfeldt W, Stols L, et al.
Appl Environ Microbiol
. 2004 Aug;
70(8):4872-9.
PMID: 15294826
Candida tropicalis (ATCC 20336) converts fatty acids to long-chain dicarboxylic acids via a pathway that includes among other reactions the oxidation of omega-hydroxy fatty acids to omega-aldehydes by a fatty...
7.
Stols L, Millard C, Dementieva I, Donnelly M
J Struct Funct Genomics
. 2004 Jul;
5(1-2):95-102.
PMID: 15263848
A simplified approach developed recently for the production of heterologous proteins in Escherichia coli uses 2-liter polyethylene terephthalate beverage bottles as disposable culture vessels [Sanville Millard, C. et al. 2003....
8.
Eschenfeldt W, Zhang Y, Samaha H, Stols L, Eirich L, Wilson C, et al.
Appl Environ Microbiol
. 2003 Oct;
69(10):5992-9.
PMID: 14532054
Candida tropicalis ATCC 20336 can grow on fatty acids or alkanes as its sole source of carbon and energy, but strains blocked in beta-oxidation convert these substrates to long-chain alpha,omega-dicarboxylic...
9.
Millard C, Stols L, Quartey P, Kim Y, Dementieva I, Donnelly M
Protein Expr Purif
. 2003 May;
29(2):311-20.
PMID: 12767824
Contemporary approaches to biology often call for the high-throughput production of large amounts of numerous proteins for structural or functional studies. Even with the highly efficient protein expression systems developed...
10.
Stols L, Gu M, Dieckman L, Raffen R, Collart F, Donnelly M
Protein Expr Purif
. 2002 Jun;
25(1):8-15.
PMID: 12071693
To establish high-throughput methods for protein crystallography, all aspects of the production and analysis of protein crystals must be accelerated. Automated, plate-based methods for cloning, expression, and evaluation of target...