Author: Koji Imamura | MedLuna.com

1.

Erratum to: High-Mannose Specific Lectin and Its Recombinants from a Carrageenophyta Kappaphycus alvarezii Represent a Potent Anti-HIV Activity Through High-Affinity Binding to the Viral Envelope Glycoprotein gp120

Hirayama M, Shibata H, Imamura K, Sakaguchi T, Hori K

Mar Biotechnol (NY) . 2016 May; 18(3):448. PMID: 27166832

No abstract available.

2.

High-Mannose Specific Lectin and Its Recombinants from a Carrageenophyta Kappaphycus alvarezii Represent a Potent Anti-HIV Activity Through High-Affinity Binding to the Viral Envelope Glycoprotein gp120

Hirayama M, Shibata H, Imamura K, Sakaguchi T, Hori K

Mar Biotechnol (NY) . 2015 Dec; 18(2):215-31. PMID: 26661793

We previously reported that a high-mannose binding lectin KAA-2 from the red alga Kappaphycus alvarezii, which is an economically important species and widely cultivated as a source of carrageenans, had...

3.

High-Mannose Specific Lectin and Its Recombinants from a Carrageenophyta Kappaphycus alvarezii Represent a Potent Anti-HIV Activity Through High-Affinity Binding to the Viral Envelope Glycoprotein gp120

Hirayama M, Shibata H, Imamura K, Sakaguchi T, Hori K

Mar Biotechnol (NY) . 2015 Nov; 18(1):144-60. PMID: 26593063

We previously reported that a high-mannose binding lectin KAA-2 from the red alga Kappaphycus alvarezii, which is an economically important species and widely cultivated as a source of carrageenans, had...

4.

Improved lectin ELISA for glycosylation analysis of biomarkers using PS-tag-fused single-chain Fv

Kumada Y, Ohigashi Y, Emori Y, Imamura K, Omura Y, Kishimoto M

J Immunol Methods . 2012 Aug; 385(1-2):15-22. PMID: 22884622

In this study, we successfully developed a novel lectin enzyme-linked immunosorbent assay (lectin ELISA) for the detection of glycosides linked to carcinoembryonic antigen (CEA) as a model antigen using a...

5.

Engineering of the glycan-binding specificity of Agrocybe cylindracea galectin towards α(2,3)-linked sialic acid by saturation mutagenesis

Imamura K, Takeuchi H, Yabe R, Tateno H, Hirabayashi J

J Biochem . 2011 Aug; 150(5):545-52. PMID: 21813503

Sialic acid represents a critical sugar component located at the outermost position of glycoconjugates, playing important roles in extensive biological processes. To date, however, there have been only few probes...

6.

Diagnosis and discrimination of autoimmune Graves' disease and Hashimoto's disease using thyroid-stimulating hormone receptor-containing recombinant proteoliposomes

Fukushima H, Matsuo H, Imamura K, Morino K, Okumura K, Tsumoto K, et al.

J Biosci Bioeng . 2009 Nov; 108(6):551-6. PMID: 19914592

Graves' disease (GD) is an autoimmune disease of the thyroid gland caused by autoantibodies against thyroid-stimulating hormone receptor (TSHR). Currently, the diagnostic test for TSHR autoantibodies is based on an...

7.

Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems

Fukushima H, Mizutani M, Imamura K, Morino K, Kobayashi J, Okumura K, et al.

J Biochem . 2008 Oct; 144(6):763-70. PMID: 18836200

We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared...

8.

Enzyme-linked immunosorbent assay using bacterial recombinant proteins of human BP230 as a diagnostic tool for bullous pemphigoid

Yoshida M, Hamada T, Amagai M, Hashimoto K, Uehara R, Yamaguchi K, et al.

J Dermatol Sci . 2005 Dec; 41(1):21-30. PMID: 16364599

Background: By immunoblot analyses of normal human epidermal extracts, the 230kDa bullous pemphigoid antigen (BP230) is recognized by most bullous pemphigoid (BP) sera. We produced different recombinant glutathione-S-transferase-fusion proteins, which...

9.

A new conformational epitope generated by the binding of recombinant 70-kd protein and U1 RNA to anti-U1 RNP autoantibodies in sera from patients with mixed connective tissue disease

Murakami A, Kojima K, Ohya K, Imamura K, Takasaki Y

Arthritis Rheum . 2002 Dec; 46(12):3273-82. PMID: 12483732

Objective: To establish an enzyme-linked immunosorbent assay (ELISA) using a complex of in vitro-transcribed U1 RNA and recombinant 70-kd, A, and C proteins (C-ELISA) to detect anti-U1 RNP antibodies reactive...