Kohei Otomo
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Explore the profile of Kohei Otomo including associated specialties, affiliations and a list of published articles.
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Articles
32
Citations
215
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Recent Articles
1.
Murata T, Tsutsumi M, Otomo K, Nemoto T
Methods Mol Biol
. 2024 Dec;
2872:37-50.
PMID: 39616567
Plant cells have a unique organization of microtubules during mitosis and cytokinesis. However, it is difficult to observe cell division in plant cells because of the distortion of images caused...
2.
Izumi M, Nakamura S, Otomo K, Ishida H, Hidema J, Nemoto T, et al.
Elife
. 2024 Nov;
12.
PMID: 39509463
Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins to facilitate efficient nutrient reuse. Here,...
3.
Otomo K, Omura T, Nozawa Y, Edwards S, Sato Y, Saito Y, et al.
Nat Commun
. 2024 Jun;
15(1):4941.
PMID: 38866781
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM...
4.
Takahashi T, Zhang H, Agetsuma M, Nabekura J, Otomo K, Okamura Y, et al.
Commun Biol
. 2024 Mar;
7(1):232.
PMID: 38438546
Two-photon microscopy enables in vivo imaging of neuronal activity in mammalian brains at high resolution. However, two-photon imaging tools for stable, long-term, and simultaneous study of multiple brain regions in...
5.
Ataka M, Otomo K, Enoki R, Ishii H, Tsutsumi M, Kozawa Y, et al.
Biomed Opt Express
. 2024 Feb;
15(2):1089-1101.
PMID: 38404301
This study presents an alternative approach for two-photon volumetric imaging that combines multibeam lateral scanning with continuous axial scanning using a confocal spinning-disk scanner and an electrically focus tunable lens....
6.
Ishii H, Otomo K, Chang C, Yamasaki M, Watanabe M, Yokoyama H, et al.
PLoS One
. 2023 Aug;
18(8):e0290550.
PMID: 37616194
Super-resolution in two-photon excitation (2PE) microscopy offers new approaches for visualizing the deep inside the brain functions at the nanoscale. In this study, we developed a novel 2PE stimulated-emission-depletion (STED)...
7.
Otomo K, Ishii H, Nemoto T
Biophys Physicobiol
. 2023 May;
20(1):e200009.
PMID: 37234851
Two-photon excitation laser scanning microscopy (TPLSM) has provided many insights into the life sciences, especially for thick biological specimens, because of its superior penetration depth and less invasiveness owing to...
8.
Otomo K, Dewa T, Matsushita M, Fujiyoshi S
J Phys Chem B
. 2023 May;
127(22):4959-4965.
PMID: 37222077
We observed the mid-infrared (MIR) response of a single pigment of bacteriochlorophyll at the B800 binding site of a light-harvesting 2 complex. At a temperature of 1.5 K, a single...
9.
Chang C, Otomo K, Kozawa Y, Ishii H, Yamasaki M, Watanabe M, et al.
Sci Rep
. 2022 Jun;
12(1):10468.
PMID: 35729283
Biological tissues and their networks frequently change dynamically across large volumes. Understanding network operations requires monitoring their activities in three dimensions (3D) with single-cell resolution. Several researchers have proposed various...
10.
Kamada T, Otomo K, Murata T, Nakata K, Hiruma S, Uehara R, et al.
Sci Rep
. 2022 Jan;
12(1):809.
PMID: 35039530
Non-linear microscopy, such as multi-photon excitation microscopy, offers spatial localities of excitations, thereby achieving 3D cross-sectional imaging with low phototoxicity even in thick biological specimens. We had developed a multi-point...