Kohei Nishimura
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Explore the profile of Kohei Nishimura including associated specialties, affiliations and a list of published articles.
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31
Citations
1434
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Recent Articles
1.
Ogawa Y, Ueda T, Obara K, Nishimura K, Kamura T
Bio Protoc
. 2024 Jul;
14(12):e5019.
PMID: 38948262
The Auxin-inducible degron (AID) system is a genetic tool that induces rapid target protein depletion in an auxin-dependent manner. Recently, two advanced AID systems-the super-sensitive AID and AID 2-were developed...
2.
Obara K, Nishimura K, Kamura T
Cells
. 2024 Feb;
13(4.
PMID: 38391905
proliferates by budding, which includes the formation of a cytoplasmic protrusion called the 'bud', into which DNA, RNA, proteins, organelles, and other materials are transported. The transport of organelles into...
3.
Ogawa Y, Ueda T, Obara K, Nishimura K, Kamura T
Cells
. 2024 Jan;
13(2).
PMID: 38247866
This review explores various methods for modulating protein stability to achieve target protein degradation, which is a crucial aspect in the study of biological processes and drug design. Thirty years...
4.
Ogawa Y, Nishimura K, Obara K, Kamura T
PLoS Genet
. 2023 Jun;
19(6):e1010731.
PMID: 37315088
Conditional control of target proteins using the auxin-inducible degron (AID) system provides a powerful tool for investigating protein function in eukaryotes. Here, we established an Affinity-linker based super-sensitive auxin-inducible degron...
5.
Obara K, Yoshikawa T, Yamaguchi R, Kuwata K, Nakatsukasa K, Nishimura K, et al.
Nat Commun
. 2022 Apr;
13(1):2005.
PMID: 35422486
In yeast, mitochondria are passed on to daughter cells via the actin cable, motor protein Myo2, and adaptor protein Mmr1. They are released from the actin-myosin machinery after reaching the...
6.
Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, et al.
Commun Biol
. 2022 Jan;
5(1):78.
PMID: 35058555
DNA transfection is an important technology in life sciences, wherein nuclear entry of DNA is necessary to express exogenous DNA. Non-viral vectors and their transfection reagents are useful as safe...
7.
Nishimura K, Fukagawa T
Methods Mol Biol
. 2021 Oct;
2377:109-122.
PMID: 34709613
Cells with a loss-of-function mutation in a gene (knockout cells) are powerful tools for characterizing the function of such gene product. However, for essential genes, conditional knockout cell lines must...
8.
Nishimura K, Fukagawa T
Bio Protoc
. 2021 Aug;
11(14):e4092.
PMID: 34395730
Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the...
9.
Takahashi N, Inagaki S, Nishimura K, Sakakibara H, Antoniadi I, Karady M, et al.
Sci Adv
. 2021 Jun;
7(25).
PMID: 34134976
Plants have a high ability to cope with changing environments and grow continuously throughout life. However, the mechanisms by which plants strike a balance between stress response and organ growth...
10.
Villa F, Fujisawa R, Ainsworth J, Nishimura K, Lie-A-Ling M, Lacaud G, et al.
EMBO Rep
. 2021 Feb;
22(3):e52164.
PMID: 33590678
The eukaryotic replisome is disassembled in each cell cycle, dependent upon ubiquitylation of the CMG helicase. Studies of Saccharomyces cerevisiae, Caenorhabditis elegans and Xenopus laevis have revealed surprising evolutionary diversity...