John Z Metcalfe
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Explore the profile of John Z Metcalfe including associated specialties, affiliations and a list of published articles.
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48
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1219
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Recent Articles
1.
Limberis J, Metcalfe J
bioRxiv
. 2025 Feb;
PMID: 39975389
Targeted sequencing of multiplex PCR amplicons is commonplace in research laboratories and clinical diagnostics. There are numerous tools for the a priori optimization of primers and reactions, but no tools...
2.
Limberis J, Nagel R, Chakravorty S, Block D, Dewell S, Nalyvayko A, et al.
bioRxiv
. 2024 Dec;
PMID: 39713367
Low-frequency mutations provide valuable insights in various fields, including drug resistance identification, cancer and infectious disease research. One promising strategy to enhance the sensitivity and specificity of mutation detection is...
3.
Limberis J, Nagel R, Chakravorty S, Block D, Dewell S, Nalyvayko A, et al.
PLoS One
. 2024 Dec;
19(12):e0314265.
PMID: 39652558
Identifying pathogens, resistance-conferring mutations, and strain types through targeted amplicon sequencing is an important tool. However, due to the limitations of short read sequencing, many applications require the division of...
4.
Limberis J, Metcalfe J
HardwareX
. 2024 Sep;
19:e00576.
PMID: 39280234
turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the...
5.
Klopper M, van der Merwe C, van der Heijden Y, Folkerts M, Loubser J, Streicher E, et al.
Ann Am Thorac Soc
. 2024 Jun;
21(10):1391-1397.
PMID: 38935769
Isoniazid-resistant tuberculosis (Hr-TB) is often overlooked in diagnostic algorithms because of reliance on first-line molecular assays testing only for rifampicin resistance. To determine the prevalence, outcomes, and molecular mechanisms associated...
6.
Metcalfe J, Economou T, Naufal F, Kucukosmanoglu M, Kleiman R, Phillips P, et al.
JAMA Netw Open
. 2024 Jun;
7(6):e2415576.
PMID: 38848063
Importance: Rifampin-resistant tuberculosis treatment regimens require electrocardiographic (ECG) monitoring due to the use of multiple QTc-prolonging agents. Formal 12-lead ECG devices represent a significant burden in resource-constrained clinics worldwide and...
7.
Limberis J, Metcalfe J
BMC Bioinformatics
. 2023 Dec;
24(1):468.
PMID: 38082220
Background: Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted...
8.
Limberis J, Nalyvayko A, Ernst J, Metcalfe J
PLoS One
. 2023 Nov;
18(11):e0289442.
PMID: 38015898
Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs...
9.
Limberis J, Nalyvayko A, Ernst J, Metcalfe J
PLoS One
. 2023 Sep;
18(9):e0288687.
PMID: 37708184
Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing,...
10.
Limberis J, Metcalfe J
Res Sq
. 2023 Jul;
PMID: 37461503
Background: Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted...