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John P Efromson

Explore the profile of John P Efromson including associated specialties, affiliations and a list of published articles. Areas
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Articles 5
Citations 47
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Recent Articles
1.
Efromson J, Li S, Lynch M
HardwareX . 2022 May; 9:e00177. PMID: 35492036
Autosampling from bioreactors reduces error, increases reproducibility and offers improved aseptic handling when compared to manual sampling. Additionally, autosampling greatly decreases the hands-on time required for a bioreactor experiment and...
2.
Menacho-Melgar R, Moreb E, Efromson J, Yang T, Hennigan J, Wang R, et al.
Biotechnol Bioeng . 2020 May; 117(9):2852-2860. PMID: 32462658
We report improved release of recombinant proteins in Escherichia coli, which relies on combined cellular autolysis and DNA/RNA autohydrolysis, conferred by the tightly controlled autoinduction of both phage lysozyme and...
3.
Menacho-Melgar R, Ye Z, Moreb E, Yang T, Efromson J, Decker J, et al.
Biotechnol Bioeng . 2020 May; 117(9):2715-2727. PMID: 32441815
We report the scalable production of recombinant proteins in Escherichia coli, reliant on tightly controlled autoinduction, triggered by phosphate depletion in the stationary phase. The method, reliant on engineered strains...
4.
Moreb E, Ye Z, Efromson J, Hennigan J, Menacho-Melgar R, Lynch M
ACS Synth Biol . 2020 May; 9(6):1483-1486. PMID: 32353228
A key challenge in synthetic biology is the successful utilization of characterized parts, such as promoters, in different biological contexts. We report the evaluation of the media robustness of a...
5.
Lebeau J, Efromson J, Lynch M
Front Bioeng Biotechnol . 2020 Apr; 8:207. PMID: 32266236
Industrial biotechnology can lead to new routes and potentially to more sustainable production of numerous chemicals. We review the potential of biobased routes from sugars to the large volume commodity,...