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Jeffrey A Smiley

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Recent Articles
1.
Hutchinson K, Bretz S, Mettee H, Smiley J
Biochem Mol Biol Educ . 2011 Jun; 33(2):123-7. PMID: 21638558
A laboratory experiment for undergraduate biophysical chemistry is described, in which the acid concentration and temperature dependences of the decarboxylation of pyrrole-2-carboxylate are measured using a continuous ultraviolet (UV) spectrophotometric...
2.
Wepukhulu W, Smiley V, Vemulapalli B, Smiley J, Phillips L, Lee J
Org Biomol Chem . 2008 Nov; 6(24):4533-41. PMID: 19039361
Orotidine-5'-monophosphate decarboxylase (OMP decarboxylase, ODCase) catalyzes the decarboxylation of orotidine-5'-monophosphate (OMP) to uridine-5'-monophosphate (UMP). Despite extensive enzymological, structural, and computational studies, the mechanism of ODCase remains incompletely characterized. Herein, carbon...
3.
Smiley J, Kundracik M, Landfried D, Barnes Sr V, Axhemi A
Biochim Biophys Acta . 2005 Mar; 1723(1-3):256-64. PMID: 15794921
Genes for two enzymes in the thymidine salvage pathway, thymine-7-hydroxylase (THase; official name thymine dioxygenase) and iso-orotate decarboxylase (IDCase) have been isolated from fungal sources. THase was isolated from a...
4.
Krungkrai S, DelFraino B, Smiley J, Prapunwattana P, Mitamura T, Horii T, et al.
Biochemistry . 2005 Feb; 44(5):1643-52. PMID: 15683248
Human malaria parasite, Plasmodium falciparum, can only synthesize pyrimidine nucleotides using the de novo pathway, whereas mammalian cells obtain pyrimidine nucleotides from both the de novo and salvage pathways. The...
5.
Smiley J, DelFraino B, Simpson B
Arch Biochem Biophys . 2003 Apr; 412(2):267-71. PMID: 12667491
The mechanism of the enzyme orotidine-5(')-monophosphate decarboxylase (OMP decarboxylase, ODCase) is not fully characterized; some of the proposed mechanisms suggest the possibility of hydrogen rearrangement (shift from C5 to C6...