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James R Duduit

Explore the profile of James R Duduit including associated specialties, affiliations and a list of published articles. Areas
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Recent Articles
1.
Brooks E, Elorriaga E, Liu Y, Duduit J, Yuan G, Tsai C, et al.
Biodes Res . 2023 Oct; 5:0013. PMID: 37849460
High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional levels. Gene transcription is tightly regulated by promoters and terminators. Promoters determine the timing, tissues and...
2.
Maren N, Duduit J, Huang D, Zhao F, Ranney T, Liu W
Methods Mol Biol . 2023 Mar; 2653:317-332. PMID: 36995635
Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used routinely to quantify gene expression levels. Primer design and the optimization of qRT-PCR parameters are critical for the accuracy and...
3.
Duduit J, Kosentka P, Miller M, Blanco-Ulate B, Lenucci M, Panthee D, et al.
Hortic Res . 2022 Jun; 9:uhac084. PMID: 35669706
Lycopene content in tomato fruit is largely under genetic control and varies greatly among genotypes. Continued improvement of lycopene content in elite varieties with conventional breeding has become challenging, in...
4.
Zhao F, Maren N, Kosentka P, Liao Y, Lu H, Duduit J, et al.
Hortic Res . 2021 Aug; 8(1):179. PMID: 34333545
Computational tool-assisted primer design for real-time reverse transcription (RT) PCR (qPCR) analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome. It can lead to...