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Jakub Dusek

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Recent Articles
1.
Fousek J, Dusek J, Hoffmeisterova H, cerovska N, Kundu J, Moravec T
Methods Mol Biol . 2024 Apr; 2787:245-253. PMID: 38656494
To properly assess promoter activity, which is critical for understanding biosynthetic pathways in different plant species, we use agroinfiltration-based transient gene expression assay. We compare the activity of several known...
2.
Moreira Da Silva C, Ortiz-Pena N, Boubekeur-Lecaque L, Dusek J, Moravec T, Alloyeau D, et al.
Nano Lett . 2023 Jun; 23(11):5281-5287. PMID: 37272864
Biotemplated syntheses have emerged as an efficient strategy to control the assembly of metal nanoparticles (NPs) and generate promising plasmonic properties for sensing or biomedical applications. However, understanding the nucleation...
3.
Plchova H, Moravec T, cerovska N, Poborilova Z, Dusek J, Kratochvilova K, et al.
Viruses . 2022 May; 14(5). PMID: 35632840
We have developed a Potato virus X (PVX)-based vector system compatible with the GoldenBraid 2.0 (GB) cloning strategy to transiently express heterologous proteins or peptides in plants for biotechnological purposes....
4.
Hoffmeisterova H, Kratochvilova K, cerovska N, Slavikova L, Dusek J, Muller K, et al.
Viruses . 2022 Feb; 14(2). PMID: 35215892
Reverse transcription PCR (RT-PCR) is a popular method for detecting RNA viruses in plants. RT-PCR is usually performed in a classical two-step procedure: in the first step, cDNA is synthesized...
5.
Dusek J, Plchova H, cerovska N, Poborilova Z, Navratil O, Kratochvilova K, et al.
Front Plant Sci . 2020 Nov; 11:522059. PMID: 33193468
Methods for simple and fast assembly of exchangeable standard DNA parts using Type II S restriction enzymes are becoming more and more popular in plant synthetic and molecular biology. These...
6.
Mercl F, Kosnar Z, Marsik P, Vojtisek M, Dusek J, Szakova J, et al.
J Hazard Mater . 2020 Nov; 405:124278. PMID: 33168310
Biosolids were applied as a fertilizer after drying, torrefaction (220, 320 °C), and pyrolysis (420, 520, 620 °C). Lettuce was grown on contrasting soils, and the transfer of pharmaceuticals to...