Jake D Johnston
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Explore the profile of Jake D Johnston including associated specialties, affiliations and a list of published articles.
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8
Citations
59
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Recent Articles
1.
Chestnut C, Johnston J, Velazquez M, Neselu K, Eng E
J Vis Exp
. 2025 Feb;
(215).
PMID: 39927669
Cryo-electron tomography (cryo-ET) is a powerful technique for visualizing the ultrastructure of cells in three dimensions (3D) at nanometer resolution. However, the manual segmentation of cellular components in cryo-ET data...
2.
Rangan R, Feathers R, Khavnekar S, Lerer A, Johnston J, Kelley R, et al.
Nat Methods
. 2024 Jul;
21(8):1537-1545.
PMID: 39025970
Advances in cryo-electron tomography (cryo-ET) have produced new opportunities to visualize the structures of dynamic macromolecules in native cellular environments. While cryo-ET can reveal structures at molecular resolution, image processing...
3.
Serbynovskyi V, Wang J, Chua E, Ishemgulova A, Alink L, Budell W, et al.
Res Sq
. 2024 Jul;
PMID: 39011111
CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. We demonstrate that vitreous ice may...
4.
Gong B, Johnston J, Thiemicke A, de Marco A, Meyer T
Nature
. 2024 Jun;
631(8020):415-423.
PMID: 38867038
Directed cell migration is driven by the front-back polarization of intracellular signalling. Receptor tyrosine kinases and other inputs activate local signals that trigger membrane protrusions at the front. Equally important...
5.
Serbynovskyi V, Wang J, Chua E, Ishemgulova A, Alink L, Budell W, et al.
bioRxiv
. 2024 Feb;
PMID: 38328036
CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. We demonstrate that vitreous ice may...
6.
Asarnow D, Becker V, Bobe D, Dubbledam C, Johnston J, Kopylov M, et al.
Front Mol Biosci
. 2024 Jan;
10:1296941.
PMID: 38288336
With the increasing spread of infectious diseases worldwide, there is an urgent need for novel strategies to combat them. Cryogenic sample electron microscopy (cryo-EM) techniques, particularly electron tomography (cryo-ET), have...
7.
Chua E, Alink L, Kopylov M, Johnston J, Eisenstein F, de Marco A
Nat Methods
. 2024 Jan;
21(4):562-565.
PMID: 38238558
Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results...
8.
Klykov O, Bobe D, Paraan M, Johnston J, Potter C, Carragher B, et al.
Bio Protoc
. 2023 Jan;
12(21).
PMID: 36618877
Cryo-focused ion beam (FIB) milling of vitrified specimens is emerging as a powerful method for in situ specimen preparation. It allows for the preservation of native and near-native conditions in...
9.
Vallese F, Kim K, Yen L, Johnston J, Noble A, Cali T, et al.
Nat Struct Mol Biol
. 2022 Jul;
29(7):706-718.
PMID: 35835865
The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its...