J Wiegant
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Explore the profile of J Wiegant including associated specialties, affiliations and a list of published articles.
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89
Citations
1725
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Recent Articles
1.
Wiegant J
Curr Protoc Cytom
. 2008 Sep;
Chapter 8:Unit 8.4.
PMID: 18770742
This unit provides detailed descriptions for direct and indirect in situ hybridization methods. Protocols are designed for bright-field microscopy using a precipitating chromogenic substrate and for immunofluorescence detection using a...
2.
Wiegant J, Raap A
Curr Protoc Cytom
. 2008 Sep;
Chapter 8:Unit 8.3.
PMID: 18770741
This unit describes in detail basic protocols for probe labeling, denaturing of in situ target DNA, in situ hybridization, and post-hybridization washes. Support protocols for probe labeling cover probe purification...
3.
Wiegant J, Raap A
Curr Protoc Cytom
. 2008 Sep;
Chapter 8:Unit 8.2.
PMID: 18770740
This unit presents protocols for preparing human metaphase chromosome slides from peripheral blood lymphocytes, isolating interphase nuclei from lymphocytes and paraffin-embedded tissues, and preparing DNA fibers. The protocols are designed...
4.
Stewenius Y, Tanke H, Wiegant J, Gisselsson D
Cytogenet Genome Res
. 2006 Sep;
114(3-4):257-62.
PMID: 16954663
Epithelial tumour karyotypes are often difficult to study by standard cytogenetic methods because of poor chromosome preparation quality and the high complexity of their genomic rearrangements. Subtelomeric fluorescence in situ...
5.
Heetebrij R, Talman E, v Velzen M, van Gijlswijk R, Snoeijers S, Schalk M, et al.
Chembiochem
. 2003 Jul;
4(7):573-83.
PMID: 12851925
The synthesis, characterization, and molecular interactions of platinum(II) coordination compounds, which contain a distal nonradioactive reporter molecule, with mono- and polynucleotides are described. A [Pt(II)(en)(NH(2)(CH(2))(6)NH-tBoc)Cl](NO(3)) (en=ethylenediamine) entity has been coupled,...
6.
Gisselsson D, Jonson T, Yu C, Martins C, Mandahl N, Wiegant J, et al.
Br J Cancer
. 2002 Jul;
87(2):202-7.
PMID: 12107843
Carcinomas of the head and neck typically exhibit complex chromosome aberrations but the underlying mutational mechanisms remain obscure. Evaluation of cell division dynamics in low-passage cell lines from three benign...
7.
Eriksson L, Mertens F, Akerman M, Wiegant J
J Oral Maxillofac Surg
. 2001 Sep;
59(10):1217-20.
PMID: 11573185
No abstract available.
8.
Gilhuis H, Bernse H, Jeuken J, Wesselin P, Sprenger S, Kerstens H, et al.
J Neurooncol
. 2001 Jun;
51(2):121-7.
PMID: 11386408
Angiogenesis is of vital importance for the growth of solid tumors and constitutes a target for anti-cancer therapy. Glioblastomas (GBMs) are histologically characterized by striking microvascular proliferation. The identification of...
9.
Fodde R, Kuipers J, Rosenberg C, Smits R, Kielman M, Gaspar C, et al.
Nat Cell Biol
. 2001 Apr;
3(4):433-8.
PMID: 11283620
Two forms of genetic instability have been described in colorectal cancer: microsatellite instability and chromosomal instability. Microsatellite instability results from mutations in mismatch repair genes; chromosomal instability is the hallmark...
10.
Jin C, Martins C, Jin Y, Wiegant J, Wennerberg J, Dictor M, et al.
Genes Chromosomes Cancer
. 2001 Jan;
30(2):161-7.
PMID: 11135432
Fluorescence in situ hybridization (FISH), including COBRA-FISH, was used to characterize 11 salivary gland tumors that had been investigated by banding analysis. Five cases were pleomorphic adenoma (PA), three were...