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J R Lakowicz

Explore the profile of J R Lakowicz including associated specialties, affiliations and a list of published articles. Areas
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Articles 283
Citations 4429
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Recent Articles
11.
Wiczk W, Eis P, Fishman M, Johnson M, Lakowicz J
J Fluoresc . 2013 Nov; 1(4):273-86. PMID: 24243077
The end-to-end distance distribution of a flexible molecule was recovered from steady-state fluorescence energy transfer measurements using the method suggested by Cantor and Pechukas (Proc. Natl. Acad. Sci. USA 68,...
12.
Gryczynski I, Szmacinski H, Laczko G, Wiczk W, Johnson M, Kusba J, et al.
J Fluoresc . 2013 Nov; 1(3):163-76. PMID: 24242994
We used gigahertz frequency-domain fluorometry to examine the tyrosyl fluorescence intensity and anisotropy decays of the single-tyrosine cyclic peptide hormones oxytocin and vasopressin. Acrylamide quenching and a distance-dependent quenching model...
13.
Lakowicz J, Gryczynski I, Laczko G, Gloyna D
J Fluoresc . 2013 Nov; 1(2):87-93. PMID: 24242958
We characterized a series of dimethylamino-stilbene derivatives as standards for time-domain and frequency-domain lifetime measurements. The substances have reasonable quantum yields, are soluble in solvents available with a high purity,...
14.
Lakowicz J, Gryczynski I, Kusba J, Danielsen E
J Fluoresc . 2013 Nov; 2(4):247-58. PMID: 24241719
We measured the fluorescence intensity and anisotropy decays of 1,6-diphenyl-1,3,5-hexatriene (DPH)-labeled membranes resulting from simultaneous two-photon excitation of fluorescence. Comparison of these two-photon data with the more usual one-photon measurements...
15.
Zelent B, Kusba J, Gryczynski I, Johnson M, Lakowicz J
J Fluoresc . 2013 Nov; 3(3):199-207. PMID: 24234834
We examined the time-dependent intensity decays ofN-acetyl-L-tryptophanamide (NATA) when collisionally quenched by acrylamide in propylene glycol over a range of temperatures. The intensity decays of NATA became increasingly heterogeneous in...
16.
Szmacinski H, Lakowicz J, Lederer W, Nowaczyk K, Johnson M
J Fluoresc . 2013 Nov; 3(3):161-7. PMID: 24234827
Fluorescence lifetime imaging microscopy (FLIM) is a new methodology for studying the spatial and temporal dynamics of macromolecule, molecules, and ions in living cells. In FLIM image contrast is derived...
17.
Terpetschnig E, Szmacinski H, Lakowicz J
J Fluoresc . 2013 Nov; 3(3):153-5. PMID: 24234825
We present an overview of our investigation of the spectral properties and photostabilities of squaraine derivatives. The objective was to identify long-wavelength probes with reasonable quantum yields, reasonably long lifetimes,...
18.
Lakowicz J, Cherek H, Kusba J, Gryczynski I, Johnson M
J Fluoresc . 2013 Nov; 3(2):103-16. PMID: 24234774
This didactic paper summarizes the mathematical expressions needed for analysis of fluorescence anisotropy decays from polarized frequency-domain fluorescence data. The observed values are the phase angle difference between the polarized...
19.
Gryczynski I, Bogdanov V, Lakowicz J
J Fluoresc . 2013 Nov; 3(2):85-92. PMID: 24234772
We observed the steady-state and time-resolved emission of tetraphenylbutadiene (TPB) whea excited by simultaneous absorption of two photons (514 to 610 nm). The intensity initially increased quadratically with laser power,...
20.
Eis P, Kusba J, Johnson M, Lakowicz J
J Fluoresc . 2013 Nov; 3(1):23-31. PMID: 24234716
Time-resolved fluorescence resonance energy transfer (FRET) measurements were used to measure distance distributions and intramolecular dynamics (site-to-site diffusion) of a 28-residue single-domain zinc finger peptide in the absence and presence...