Two Photon-induced Fluorescence Intensity and Anisotropy Decays of Diphenylhexatriene in Solvents and Lipid Bilayers

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2013 Nov 19

PMID 24241719

Citations 14

Authors

J R Lakowicz

I Gryczynski

J Kusba

E Danielsen

Affiliations

Soon will be listed here.

Abstract

We measured the fluorescence intensity and anisotropy decays of 1,6-diphenyl-1,3,5-hexatriene (DPH)-labeled membranes resulting from simultaneous two-photon excitation of fluorescence. Comparison of these two-photon data with the more usual one-photon measurements revealed that DPH displayed identical intensity decays, anisotropy decays, and order parameters for one- and two-photon excitation. While the anisotropy data are numerically distinct, they can be compared by use of the factor 10/7, which accounts for the two-photon versus one-photon photoselection. The increased time 0 anisotropy of DPH can result in increased resolution of complex anisotropy decays. Global analysis of the one- and two-photon data reveals consistency with a single apparent angle between the absorption and the emission oscillators. The global anisotropy analysis also suggests that, except for the photoselection factor, the anisotropy decays are the same for one-and two-photon excitation. This ideal behavior of DPH as a two-photon absorber, and its high two-photon cross section, makes DPH a potential probe for confocal two-photon microscopy and other systems where it is advantageous to use long-wavelength (680- to 760-nm) excitation.

Citing Articles

Light Quenching of Fluorescence Using Time-Delayed Laser Pulses As Observed by Frequency-Domain Fluorometry.

Gryczynski I, Kusba J, Lakowicz J J Phys Chem. 2020; 98(36):8886-8895.

PMID: 31902951 PMC: 6941149. DOI: 10.1021/j100087a012.

Fluorescence Spectral Properties of 2,5-Diphenyl-1,3,4-oxadiazole with Two-Color Two-Photon Excitation.

Lakowicz J, Gryczynski I, Malak H, Gryczynski Z J Phys Chem. 2019; 100(50):19406-19411.

PMID: 31849370 PMC: 6917482. DOI: 10.1021/jp962114w.

ADVANCES IN FLUORESCENCE SPECTROSCOPY: MULTI-PHOTON EXCITATION, ENGINEERED PROTEINS, MODULATION SENSING AND MICROSECOND RHENIUM METAL-LIGAND COMPLEXES.

Lakowicz J, Gryczynski I, Tolosa L, Dattelbaum J, Castellano F, Li L Acta Phys Pol A. 2019; 95(1):179-195.

PMID: 31660002 PMC: 6816252. DOI: 10.12693/APhysPolA.95.179.

Fluorescence anisotropy imaging in drug discovery.

Vinegoni C, Fumene Feruglio P, Gryczynski I, Mazitschek R, Weissleder R Adv Drug Deliv Rev. 2018; 151-152:262-288.

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Fluorescence intensity and anisotropy decays of the DNA stain Hoechst 33342 resulting from one-photon and two-photon excitation.

Gryczynski I, Lakowicz J J Fluoresc. 2013; 4(4):331-6.

PMID: 24233609 DOI: 10.1007/BF01881450.

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