J Porath
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Explore the profile of J Porath including associated specialties, affiliations and a list of published articles.
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142
Citations
1843
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Recent Articles
1.
Berna P, Berna N, Porath J, Oscarsson S
J Chromatogr A
. 1998 Apr;
800(2):151-9.
PMID: 9561759
Protein adsorption of human serum onto six different agarose-based chromatographic gels that were representative of the salt-promoted adsorbent family [octyl- and phenyl-Sepharose, mercaptoethanol-divinyl sulfone agarose (T gel), mercaptomethylene pyridine-derivatized agarose...
2.
Porath J
J Protein Chem
. 1997 Jul;
16(5):463-8.
PMID: 9246630
Adsorption and size exclusion in starch and cross-linked dextran were phenomena discovered in Uppsala in the 1950s [Porath (1979), Biochem. Soc. Trans. 7, 1197; Porath (1981), Current Content 19, 21;...
3.
Berna P, Mrabet N, Van Beeumen J, Devreese B, Porath J, Vijayalakshmi M
Biochemistry
. 1997 Jun;
36(23):6896-905.
PMID: 9188684
Subspecies defining the maturation pathway of bovine chymotrypsinogen to alpha-chymotrypsin have been separated in a single chromatographic run by affinity to iminodiacetic acid-Cu(II) [IDA-Cu(II)] immobilized onto Novarose. A major highlight...
4.
Berna P, Porath J
J Chromatogr B Biomed Sci Appl
. 1997 Jun;
693(2):277-85.
PMID: 9210430
Electron donor acceptor gels based on cyanocarbons have been tested for human serum protein adsorption in the absence of salt-promotion by water-structuring salt. This phenomenon was compared with a normal...
5.
Berna P, Porath J
J Chromatogr A
. 1996 Nov;
753(1):57-62.
PMID: 8962505
The adsorption of human serum proteins to mono-, di- and tri-cyanocarbon ligands was studied in the presence of the water-structuring salt Na2SO4. All of the ligands adsorbed proteins to varying...
6.
Winzerling J, Pham D, Kunz S, Law J, Porath J
J Mol Recognit
. 1996 Sep;
9(5-6):747.
PMID: 9174967
Vertebrate transferrin is a well characterized iron transport protein. In contrast, little is known concerning the role of transferrin in insects. Yet, study of iron metabolism in insects could give...
7.
Ehteshami G, Porath J, Guzman R
J Mol Recognit
. 1996 Sep;
9(5-6):733-7.
PMID: 9174965
The interaction of immobilized metal-chelating adsorbents with a dual heterobifunctional soluble polyethylene glycol (PEG) of the form X-PEG-Y is described, where X represents an affinity ligand and Y a chelating...
8.
Winzerling J, Pham D, Kunz S, Samaraweera P, Law J, Porath J
Protein Expr Purif
. 1996 Mar;
7(2):137-42.
PMID: 8812846
We report the purification of secreted recombinant Manduca sexta transferrin from Spodoptera frugiperda (Sf9) cell culture medium in a single step using high capacity Ni(2+)-dipicolylamine (DPA)-Novarose gel. Although the original...
9.
Rapid one-step purification of goat immunoglobulins by immobilized metal ion affinity chromatography
Boden V, Winzerling J, Vijayalakshmi M, Porath J
J Immunol Methods
. 1995 Apr;
181(2):225-32.
PMID: 7745251
A rapid, single step purification of immunoglobulins from goat serum was achieved using immobilized metal ion affinity chromatography (IMAC) on a new high capacity gel, Novarose, coupled to tris(2-aminoethyl)amine (TREN)...
10.
Winzerling J, Nez P, Porath J, Law J
Insect Biochem Mol Biol
. 1995 Feb;
25(2):217-24.
PMID: 7711752
We report methods for the rapid purification of two iron-binding proteins from larval hemolymph of Manduca sexta. Ferritin was purified in two steps by density gradient ultracentrifugation. To accomplish this,...