J A Steinkamp
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Explore the profile of J A Steinkamp including associated specialties, affiliations and a list of published articles.
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61
Citations
631
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Recent Articles
1.
Deka C, Steinkamp J
Appl Opt
. 2010 Nov;
35(22):4481-9.
PMID: 21102863
A novel method is described for the measurement and analysis of fluorescence decays of individual cells and particles in flow. It combines the rapid measurement capabilities of a flow cytometer...
2.
Steinkamp J
Curr Protoc Cytom
. 2008 Sep;
Chapter 1:Unit 1.15.
PMID: 18770664
This unit provides a detailed review and technical discussion of the principles of time-resolved fluorescence. The unique feature of this particular unit is that the principles are directly tied to...
3.
Crissman H, Steinkamp J
Methods Cell Biol
. 2000 Nov;
63:131-48.
PMID: 11060839
No abstract available.
4.
Keij J, Steinkamp J
Cytometry
. 2000 Feb;
39(3):203-10.
PMID: 10685077
Background: We set out to develop an assay for the simultaneous analysis of mitochondrial membrane potential and mass using the probes 10-nonyl acridine orange (NAO), MitoFluor Green (MFG), and MitoTracker...
5.
Steinkamp J, Valdez Y, Lehnert B
Cytometry
. 2000 Feb;
39(1):45-55.
PMID: 10655562
Background: Spectral interference (overlap) from phagocytosed green-yellow (GY) microspheres in the flow cytometric, red fluorescence emission measurement channel causes errors in quantifying damaged/dead alveolar macrophages by uptake of propidium iodide....
6.
Keij J, Steinkamp J
Cytometry
. 1999 Nov;
35(1):48-54.
PMID: 10554180
Background: Analysis of the DNA cell cycle and glutathione content cannot be performed on viable cells, because the fluorescence emissions of the DNA-specific probe Hoechst 33342 and the glutathione-specific probe...
7.
Steinkamp J, Lehnert N, Keij J, Lehnert B
Cytometry
. 1999 Nov;
37(4):275-83.
PMID: 10547612
Background: The primary source of interference in immunofluorescence measurements by flow cytometry is background autofluorescence. Methods: Using human lung fibroblasts (HLFs) as an autofluorescent cell model, unfixed HLFs and HLFs...
8.
Steinkamp J, Lehnert B, Lehnert N
J Immunol Methods
. 1999 Jul;
226(1-2):59-70.
PMID: 10410972
We report a flow cytometric fluorescence lifetime-based method to discriminate damaged/dead from viable cells in immunofluorescently labeled populations using propidium iodide as a dye-exclusion viability probe. Fluorescence signals from propidium...
9.
Sailer B, Steinkamp J, Crissman H
Eur J Histochem
. 1999 Mar;
42 Spec No:19-27.
PMID: 10076766
A new dimension has been added to multiparameter flow cytometric analysis through the recent development of techniques for rapidly measuring the fluorescence lifetime of probes bound to single cells. The...
10.
Keij J, Steinkamp J
Cytometry
. 1998 Nov;
33(3):318-23.
PMID: 9822342
FlowMetrix (Luminex, Austin, TX) microspheres were recently introduced as a platform for bead-based assays involving antibodies, enzymes, toxins, and nucleic acids. The procedure involves classification of the microspheres by their...