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I Rayment

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Articles 103
Citations 4649
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Recent Articles
1.
Abad-Zapatero C, Abdel-Meguid S, Johnson J, Leslie A, Rayment I, Rossmann M, et al.
Nature . 2009 Aug; 286(5768):33-9. PMID: 19711553
X-ray diffraction studies reveal that the polypeptide chain of the southern bean mosaic virus protein subunit has a fold closely similar to the shell domain of tomato bushy stunt virus....
2.
Rocco C, Dennison K, Klenchin V, Rayment I, Escalante-Semerena J
Plasmid . 2008 Feb; 59(3):231-7. PMID: 18295882
We describe the construction and use of two sets of vectors for the over-expression and purification of protein from Escherichia coli. The set of pTEV plasmids (pTEV3, 4, 5) directs...
3.
Allingham J, Klenchin V, Rayment I
Cell Mol Life Sci . 2006 Aug; 63(18):2119-34. PMID: 16909206
Natural small-molecule inhibitors of actin cytoskeleton dynamics have long been recognized as valuable molecular probes for dissecting complex mechanisms of cellular function. More recently, their potential use as chemotherapeutic drugs...
4.
Grimek T, HOLDEN H, Rayment I, Escalante-Semerena J
J Bacteriol . 2003 Aug; 185(16):4837-43. PMID: 12897003
The prpB gene of Salmonella enterica serovar Typhimurium LT2 encodes a protein with 2-methylisocitrate (2-MIC) lyase activity, which cleaves 2-MIC into pyruvate and succinate during the conversion of propionate to...
5.
Gulick A, Schmidt D, Gerlt J, Rayment I
Biochemistry . 2001 Dec; 40(51):15716-24. PMID: 11747448
The members of the enolase superfamily catalyze different overall reactions, yet share a partial reaction that involves Mg(2+)-assisted enolization of the substrate carboxylate anion. The fate of the resulting enolate...
6.
Gulick A, Hubbard B, Gerlt J, Rayment I
Biochemistry . 2001 Aug; 40(34):10054-62. PMID: 11513584
D-Glucarate dehydratase from Escherichia coli (GlucD), a member of the enolase superfamily, catalyzes the dehydration of both D-glucarate and L-idarate to form 5-keto-4-deoxy-D-glucarate (KDG). Previous mutagenesis and structural studies identified...
7.
Cheong C, Escalante-Semerena J, Rayment I
J Biol Chem . 2001 Jul; 276(40):37612-20. PMID: 11441022
Nicotinate mononucleotide (NaMN):5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella enterica plays a central role in the synthesis of alpha-ribazole, a key component of the lower ligand of cobalamin. Surprisingly, CobT can phosphoribosylate...
8.
BAUER C, Fonseca M, Holden H, Thoden J, Thompson T, Escalante-Semerena J, et al.
Biochemistry . 2001 Jan; 40(2):361-74. PMID: 11148030
In Salmonella typhimurium, formation of the cobalt-carbon bond in the biosynthetic pathway for adenosylcobalamin is catalyzed by the product of the cobA gene which encodes a protein of 196 amino...
9.
Thompson T, Garrett J, Taylor E, Meganathan R, Gerlt J, Rayment I
Biochemistry . 2000 Sep; 39(35):10662-76. PMID: 10978150
The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution,...
10.
BAUER C, Holden H, Thoden J, Smith R, Rayment I
J Biol Chem . 2000 Aug; 275(49):38494-9. PMID: 10954715
Myosin is the most comprehensively studied molecular motor that converts energy from the hydrolysis of MgATP into directed movement. Its motile cycle consists of a sequential series of interactions between...