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H P Too

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Articles 28
Citations 187
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Recent Articles
1.
Ho Y, Too H
Gene Ther . 2020 Apr; 27(10-11):482-494. PMID: 32341483
Transfection of surface adherent cells remain as a standard methodology for lentiviral production for early phase clinical studies and research purposes. Production today is based on transient co-transfection of three...
2.
Wu J, Zheng H, Cheng H, Zhou L, Leong K, Rajagopalan R, et al.
Langmuir . 2014 Feb; 30(8):2206-15. PMID: 24528207
We present the results of a systematic study on the porosity of silica microparticles and nanowires prepared by glancing angle deposition-metal-assisted chemical etching (GLAD-MACE) and interference lithography-metal-assisted chemical etching (IL-MACE)...
3.
Dawood M, Zhou L, Zheng H, Cheng H, Wan G, Rajagopalan R, et al.
Lab Chip . 2012 Oct; 12(23):5016-24. PMID: 23081694
We demonstrate the fabrication of a novel platform based on Si nanowire arrays integrated with a programmable DNA-directed homogeneous-phase analyte-capture strategy for robust detection of bio-analytes. The nanofabrication process used,...
4.
Lim Q, Zhou L, Ho Y, Wan G, Too H
Neuroscience . 2011 Nov; 199:32-43. PMID: 22051575
Accurate profiling of microRNAs (miRNAs) is an essential step for understanding the functional significance of these small RNAs in both physiological and pathological processes. Quantitative real-time PCR (qPCR) has gained...
5.
Zhu M, Zhou L, Li B, Dawood M, Wan G, Lai C, et al.
Nanoscale . 2011 Apr; 3(7):2723-9. PMID: 21483976
Emerging evidence of the striking differences that can be induced in the behavior of biological cells through topographical modulation of physically and chemically patterned nanostructured surfaces provides a great impetus...
6.
He E, Yue C, Simeon F, Zhou L, Too H, Tam K
J Biomed Mater Res A . 2008 Dec; 91(3):708-18. PMID: 19048636
Amphiphilic polyelectrolytes comprising cationic and uncharged hydrophilic segments condensed negatively charged DNA to form a core-shell structure stabilized by a layer of hydrophilic corona chains. At physiological pH, four-arm star-shaped...
7.
Tan J, Hatton T, Tam K, Too H
Biomacromolecules . 2007 Feb; 8(2):448-54. PMID: 17291068
Transfection efficiencies of several polymeric gene carriers were compared and correlated quantitatively to the amounts of cellular accumulation of plasmid DNA and to the expression of mRNA by quantitative real-time...
8.
Tan J, Too H, Hatton T, Tam K
Langmuir . 2006 Apr; 22(8):3744-50. PMID: 16584251
The aggregation behavior and the thermodynamics of binding between poly(ethylene oxide)-block-poly(2-(diethylamino)ethyl methacrylate) (PEO-b-PDEAEMA) block copolymers and plasmid DNA were examined. Binding between the polymer and DNA were confirmed by gel...
9.
Yin H, Too H, Chow G
Biomaterials . 2005 Jun; 26(29):5818-26. PMID: 15949547
The safety and toxicity of nanoparticles are of growing concern despite their significant scientific interests and promising potentials in many applications. The properties of nanoparticles depend not only on the...
10.
Tan J, Ravi P, Too H, Hatton T, Tam K
Biomacromolecules . 2005 Jan; 6(1):498-506. PMID: 15638558
Biotinylated and non-biotinylated copolymers of poly(ethylene oxide) (PEO) and poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) were synthesized by the atom transfer radical polymerization technique. The chemical compositions of the copolymers as determined by...