Eiji Usukura
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Explore the profile of Eiji Usukura including associated specialties, affiliations and a list of published articles.
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10
Citations
64
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Recent Articles
1.
Holz D, Hall A, Usukura E, Yamashiro S, Watanabe N, Vavylonis D
Elife
. 2022 Jun;
11.
PMID: 35670664
Single molecule imaging has shown that part of actin disassembles within a few seconds after incorporation into the dendritic filament network in lamellipodia, suggestive of frequent destabilization near barbed ends....
2.
Usukura J, Narita A, Matsumoto T, Usukura E, Sunaoshi T, Watanabe S, et al.
Sci Rep
. 2021 Nov;
11(1):21406.
PMID: 34725450
The scanning electron microscope (SEM) has been reassembled into a new type of cryo-electron microscope (cryo-TSEM) by installing a new cryo-transfer holder and anti-contamination trap, which allowed simultaneous acquisition of...
3.
Morone N, Usukura E, Narita A, Usukura J
Microscopy (Oxf)
. 2020 May;
69(6):350-359.
PMID: 32447402
Unroofing, which is the mechanical shearing of a cell to expose the cytoplasmic surface of the cell membrane, is a unique preparation method that allows membrane cytoskeletons to be observed...
4.
Usukura E, Yanase Y, Ishijima A, Kuboki T, Kidoaki S, Okamoto K, et al.
PLoS One
. 2017 Dec;
12(12):e0189708.
PMID: 29244869
This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR...
5.
Usukura E, Narita A, Yagi A, Sakai N, Uekusa Y, Imaoka Y, et al.
Sci Rep
. 2017 Jul;
7(1):6462.
PMID: 28743939
The use of cryosectioning facilitates the morphological analysis and immunocytochemistry of cells in tissues in atomic force microscopy (AFM). The cantilever can access all parts of a tissue sample in...
6.
Masuda S, Yanase Y, Usukura E, Ryuzaki S, Wang P, Okamoto K, et al.
Sci Rep
. 2017 Jun;
7(1):3720.
PMID: 28623338
This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled...
7.
Makihara M, Watanabe T, Usukura E, Kaibuchi K, Narita A, Tanaka N, et al.
Microscopy (Oxf)
. 2016 Sep;
65(6):488-498.
PMID: 27587510
An improved unroofing method consisting of tearing off the cell membrane using an adhesive electron microscopy (EM) grid instead of vitreous ice sectioning (cryo-sectioning) has enabled us to panoramically view...
8.
Usukura E, Narita A, Yagi A, Ito S, Usukura J
Sci Rep
. 2016 Jun;
6:27472.
PMID: 27273367
An improved unroofing method enabled the cantilever of an atomic force microscope (AFM) to reach directly into a cell to visualize the intracellular cytoskeletal actin filaments, microtubules, clathrin coats, and...
9.
Narita A, Usukura E, Yagi A, Tateyama K, Akizuki S, Kikumoto M, et al.
Microscopy (Oxf)
. 2016 Jun;
65(4):370-7.
PMID: 27242058
Actin filaments, the actin-myosin complex and the actin-tropomyosin complex were observed by a tip-scan atomic force microscope (AFM), which was recently developed by Olympus as the AFM part of a...
10.
Usukura E, Suzuki T, Furuike S, Soga N, Saita E, Hisabori T, et al.
J Biol Chem
. 2011 Dec;
287(3):1884-91.
PMID: 22128167
ATP synthase (F(0)F(1)) is made of two motors, a proton-driven motor (F(0)) and an ATP-driven motor (F(1)), connected by a common rotary shaft, and catalyzes proton flow-driven ATP synthesis and...