Douglas E Chandler
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Explore the profile of Douglas E Chandler including associated specialties, affiliations and a list of published articles.
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Articles
13
Citations
63
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Recent Articles
1.
Chandler D, Vacquier V
Dev Growth Differ
. 2023 Jun;
30(1):49-59.
PMID: 37281721
Immediately after fertilization sea urchin eggs undergo an increase in cytoplasmic pH from 6.8 to 7.2. This pH change occurs by activation of a Na /H antiporter, and is a...
2.
Abraham A, Chandler D
J Mol Evol
. 2017 Oct;
85(3-4):137-157.
PMID: 29071358
Proteins of the CAP superfamily play numerous roles in reproduction, innate immune responses, cancer biology, and venom toxicology. Here we document the breadth of the CAP (Cysteine-RIch Secretory Protein (CRISP),...
3.
Chandler D, Sharp W
Methods Mol Biol
. 2013 Dec;
1117:95-132.
PMID: 24357361
Freeze fracture depends on the property of frozen tissues or cells, when cracked open, to split along the hydrophobic interior of membranes, thus revealing broad panoramas of membrane interior. These...
4.
Sugiyama H, Chandler D
Protoplasma
. 2013 Oct;
251(3):461-75.
PMID: 24085342
Sperm respond to multiple cues during guidance to the egg including chemical attractants, temperature, and fluid flow. Of these, sperm chemotaxis has been studied most extensively-over 100 years-but only recently...
5.
Burnett L, Washburn C, Sugiyama H, Xiang X, Olson J, Al-Anzi B, et al.
Int Rev Cell Mol Biol
. 2012 Mar;
295:1-61.
PMID: 22449486
Eggs of many species are surrounded by extracellular coats that emit ligands to which conspecific sperm respond by undergoing chemotaxis and changes in metabolism, motility, and acrosomal status in preparation...
6.
Burnett L, Tholl N, Chandler D
J Vis Exp
. 2012 Jan;
(58):e3407.
PMID: 22231741
Sperm chemoattraction in invertebrates can be sufficiently robust that one can place a pipette containing the attractive peptide into a sperm suspension and microscopically visualize sperm accumulation around the pipette....
7.
Burnett L, Anderson D, Rawls A, Bieber A, Chandler D
Dev Biol
. 2011 Oct;
360(2):318-28.
PMID: 22008793
Allurin, a 21 kDa protein isolated from egg jelly of the frog Xenopus laevis, has previously been demonstrated to attract frog sperm in two-chamber and microscopic assays. cDNA cloning and...
8.
Tholl N, Naqvi S, McLaughlin E, Boyles S, Bieber A, Chandler D
Biol Bull
. 2011 Jun;
220(3):174-85.
PMID: 21712226
The motility of Xenopus sperm is initiated by the osmotic shock experienced when these cells are ejaculated into low-salinity pond water. Motility is brief and is required for the sperm...
9.
Burnett L, Sugiyama H, Bieber A, Chandler D
Mol Reprod Dev
. 2011 Jun;
78(6):450-62.
PMID: 21692128
Previously we have shown that extracts from Xenopus egg jelly (egg water) increase the passage of sperm through a porous membrane in a dose-dependent manner. Although this assay has shown...
10.
Sugiyama H, Burnett L, Xiang X, Olson J, Willis S, Miao A, et al.
Mol Reprod Dev
. 2008 Oct;
76(6):527-36.
PMID: 18951371
Allurin, a sperm chemoattractant isolated from Xenopus laevis egg jelly, can be purified in one step from an extract of diffusible jelly proteins ("egg water") using a FPLC or HPLC...