Douglas D Randall
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Explore the profile of Douglas D Randall including associated specialties, affiliations and a list of published articles.
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10
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Recent Articles
1.
Broz A, Tovar-Mendez A, Mooney B, Johnston M, Miernyk J, Randall D
Mitochondrion
. 2014 May;
19 Pt B:144-53.
PMID: 24846799
The Arabidopsis thaliana genome includes three genes for mitochondrial dihydrolipoamide acetyltransferase, the E2-component of the mitochondrial pyruvate dehydrogenase complex (PDC). Two genes encode E2-proteins with a single lipoyl domain, while...
2.
Tovar-Mendez A, Miernyk J, Hoyos E, Randall D
Protoplasma
. 2013 Jul;
251(1):265-71.
PMID: 23832523
In the reference dicot plant Arabidopsis thaliana, the PP2C family of P-protein phosphatases includes the products of 80 genes that have been separated into ten multi-protein clades plus six singletons....
3.
Hirani T, Tovar-Mendez A, Miernyk J, Randall D
Enzyme Res
. 2011 Feb;
2011:939068.
PMID: 21318135
We have developed an in vitro system for detailed analysis of reversible phosphorylation of the plant mitochondrial pyruvate dehydrogenase complex, comprising recombinant Arabidopsis thalianaα2β2-heterotetrameric pyruvate dehydrogenase (E1) plus A. thaliana...
4.
Huang Y, Houston N, Tovar-Mendez A, Stevenson S, Miernyk J, Randall D, et al.
Anal Biochem
. 2010 Mar;
402(1):69-76.
PMID: 20346904
The Homo sapiens and Arabidopsis thaliana genomes are believed to encode more than 500 and 1000 protein kinases, respectively. Despite this abundance, few bona fide kinase-client relationships have been described...
5.
Tovar-Mendez A, Hirani T, Miernyk J, Randall D
Arch Biochem Biophys
. 2005 Jan;
434(1):159-68.
PMID: 15629119
It has been proposed that "Glu238" within the N-box of pyruvate dehydrogenase kinase (PDK) is a base catalyst. The pH dependence of k(cat) of Arabidopsis thaliana PDK indicates that ionizable...
6.
Szurmak B, Strokovskaya L, Mooney B, Randall D, Miernyk J
Protein Expr Purif
. 2003 Apr;
28(2):357-61.
PMID: 12699701
A vector was constructed for expression of Arabidopsis thaliana mitochondrial pyruvate dehydrogenase (E1) in the cytoplasm of Trichoplusia ni cells. The construct pDDR101 comprises the mature-E1alpha coding sequence under control...
7.
Tovar-Mendez A, Miernyk J, Randall D
Eur J Biochem
. 2003 Mar;
270(6):1043-9.
PMID: 12631264
The pyruvate dehydrogenase complex (PDC) is subjected to multiple interacting levels of control in plant cells. The first level is subcellular compartmentation. Plant cells are unique in having two distinct,...
8.
Mooney B, Miernyk J, Randall D
Annu Rev Plant Biol
. 2002 Sep;
53:357-75.
PMID: 12221980
Plant cells are unique in that they contain four species of alpha-ketoacid dehydrogenase complex: plastidial pyruvate dehydrogenase, mitochondrial pyruvate dehydrogenase, alpha-ketoglutarate (2-oxoglutarate) dehydrogenase, and branched-chain alpha-ketoacid dehydrogenase. All complexes include...
9.
Tovar-Mendez A, Miernyk J, Randall D
Eur J Biochem
. 2002 May;
269(10):2601-6.
PMID: 12027899
Pyruvate dehydrogenase kinase (PDK) is the primary regulator of flux through the mitochondrial pyruvate dehydrogenase complex (PDC). Analysis of the primary amino-acid sequences of PDK from various sources reveals that...
10.
Luethy M, Gemel J, Johnston M, Mooney B, Miernyk J, Randall D
Physiol Plant
. 2001 Jul;
112(4):559-566.
PMID: 11473717
In order to better understand control of the mitochondrial pyruvate dehydrogenase complex (PDC), total catalytic activity was determined during development of the primary leaves of pea (Pisum sativum L.) seedlings,...