Darren N Nesbeth
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Explore the profile of Darren N Nesbeth including associated specialties, affiliations and a list of published articles.
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22
Citations
89
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Recent Articles
1.
Howe G, Bal M, Wasmuth M, Massaro G, Rahim A, Ali S, et al.
Mol Ther Methods Clin Dev
. 2024 Sep;
32(3):101317.
PMID: 39257529
We sought to engineer mammalian cells to secrete nuclease activity as a step toward removing the need to purchase commercial nucleases as process additions in bioprocessing of AAV5 and AAV9...
2.
Redwood-Sawyerr C, Howe G, Evans Theodore A, Nesbeth D
ACS Synth Biol
. 2024 Sep;
13(10):3163-3172.
PMID: 39240234
HeLa cell transfection with plasmid DNA (pDNA) is widely used to materialize biologicals and as a preclinical test of nucleic acid-based vaccine efficacy. We sought to genetically encode mammalian transfection...
3.
Wilbourn B, Nesbeth D, Wainwright L, Field M
Biochem J
. 2024 Apr;
481(7):565-566.
PMID: 38597906
No abstract available.
4.
Howe G, Wasmuth M, Emanuelle P, Massaro G, Rahim A, Ali S, et al.
ACS Synth Biol
. 2024 Jan;
13(2):466-473.
PMID: 38266181
We engineered HEK293T cells with a transgene encoding tetracycline-inducible expression of a nuclease incorporating a translocation signal. We adapted the unmodified and nuclease-engineered cell lines to grow in suspension in...
5.
Ali S, Rivera M, Ward J, Keshavarz-Moore E, Mason C, Nesbeth D
Heliyon
. 2023 Jul;
9(6):e17067.
PMID: 37484388
At present lentiviral vector production for cell and gene therapy commonly involves transient plasmid transfection of mammalian cells cultivated in serum-containing media and addition of exogenous nuclease to reduce host...
6.
Redwood-Sawyerr C, Nesbeth D
Biol Open
. 2023 Jun;
12(7).
PMID: 37284818
We report data consistent with tetracycline-mediated fluorescence having the potential to be an effective marker of senescence in immortalised cells. HeLa cells that had previously undergone more than 20 passages...
7.
Bandyopadhyay S, Pavlika V, Bracewell D, Nesbeth D
ACS Synth Biol
. 2023 Feb;
12(3):657-663.
PMID: 36848292
We constructed a three-input biological logic gate: S OR (G XNOR M), where S is sorbitol, G is glycerol, and M is methanol, to optimize co-expression of two transgenes in...
8.
Borg Y, Alsford S, Pavlika V, Zaikin A, Nesbeth D
Heliyon
. 2022 Feb;
8(2):e08891.
PMID: 35198764
Kinetoplastid protozoa possess properties that are highly divergent from the mammalian, yeast and bacterial cells more commonly used in synthetic biology and represent a tantalisingly untapped source of bioengineering potential....
9.
Panzella L, Benning K, Nesbeth D, Setaro B, DErrico G, Napolitano A, et al.
Food Chem
. 2021 Nov;
373(Pt B):131474.
PMID: 34731814
Reported herein is the purification of the pigment of black sturgeon caviar and its unambiguous identification as a typical eumelanin by means of chemical degradation coupled with electron paramagnetic resonance...
10.
Braun-Galleani S, Henriquez M, Nesbeth D
Heliyon
. 2019 Aug;
5(8):e02338.
PMID: 31467995
We have engineered strain BG-10 of the methylotrophic yeast for use as an effective whole cell biocatalyst. We introduced into the yeast a transgene encoding a ω-transaminase for transcription in...