Daniel Degreif
Overview
Explore the profile of Daniel Degreif including associated specialties, affiliations and a list of published articles.
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7
Citations
43
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Recent Articles
1.
Holer S, Degreif D, Stix F, Yang S, Gao S, Nagel G, et al.
PLoS One
. 2023 Apr;
18(4):e0280711.
PMID: 37053213
Channelrhodopsin 2 (ChR2) and its variants are the most frequent tools for remote manipulation of electrical properties in cells via light. Ongoing attempts try to enlarge their functional spectrum with...
2.
Mund M, Weber W, Degreif D, Schiklenk C
Microb Biotechnol
. 2023 Mar;
16(5):1000-1010.
PMID: 36929689
A broad variety of biomolecules is industrially produced in bacteria and yeasts. These microbial expression hosts can be optimized through genetic engineering using CRISPR tools. Here, we designed and characterized...
3.
Holer S, Bertl A, Degreif D
FEMS Yeast Res
. 2021 Jul;
21(5).
PMID: 34232310
The most widely used strategy for selection of yeast transformed with episomal plasmids comprises the use of auxotrophic yeast strains in combination with vectors containing complementing prototrophic marker genes. Another...
4.
Degreif D, Kremenovic M, Geiger T, Bertl A
J Biol Methods
. 2019 Aug;
5(3):e98.
PMID: 31453248
The CRISPR/Cas9 technology has greatly improved genome editing in over recent years. However, several current CRISPR/Cas9 systems suffer from work-intensive cloning procedures and/or the requirement of co-transforming target cells with...
5.
Degreif D, Cucu B, Budin I, Thiel G, Bertl A
Biochim Biophys Acta Mol Cell Biol Lipids
. 2019 Mar;
1864(7):1005-1016.
PMID: 30917917
Cells maintain physicochemical characteristics of membranes in order to allow for proper function of membrane-associated cellular processes, such as endocytosis and exocytosis. To investigate the interplay between membrane properties and...
6.
Cucu B, Degreif D, Bertl A, Thiel G
Cell Calcium
. 2017 Oct;
67:40-45.
PMID: 29029789
Measurements of the membrane capacitance on animal cells has provided an excellent technique for monitoring of exo- and endocytotic activity in intact living cells. Here we review recent data in...
7.
Degreif D, de Rond T, Bertl A, Keasling J, Budin I
Metab Eng
. 2017 Mar;
41:46-56.
PMID: 28323063
Cells modulate lipid metabolism in order to maintain membrane homeostasis. Here we use a metabolic engineering approach to manipulate the stoichiometry of fatty acid unsaturation, a regulator of cell membrane...