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Bhaskar Malayappan

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Recent Articles
1.
Hill R, Oman T, Wang X, Shan G, Schafer B, Herman R, et al.
J Agric Food Chem . 2017 Jun; 65(27):5531-5544. PMID: 28635260
As part of the regulatory approval process in Europe, comparison of endogenous soybean allergen levels between genetically engineered (GE) and non-GE plants has been requested. A quantitative multiplex analytical method...
2.
Chesner L, Degner A, Sangaraju D, Yomtoubian S, Wickramaratne S, Malayappan B, et al.
Int J Mol Sci . 2017 May; 18(5). PMID: 28524082
Xenobiotic-induced interstrand DNA-DNA cross-links (ICL) interfere with transcription and replication and can be converted to toxic DNA double strand breaks. In this work, we investigated cellular responses to 1,4--(guan-7-yl)-2,3-butanediol (-N7G-BD)...
3.
Wickramaratne S, Banda D, Ji S, Manlove A, Malayappan B, Nunez N, et al.
Biochemistry . 2016 Aug; 55(43):6070-6081. PMID: 27552084
The important industrial and environmental carcinogen 1,3-butadiene (BD) forms a range of adenine adducts in DNA, including N-(2-hydroxy-3-buten-1-yl)-2'-deoxyadenosine (N-HB-dA), 1,N-(2-hydroxy-3-hydroxymethylpropan-1,3-diyl)-2'-deoxyadenosine (1,N-HMHP-dA), and N,N-(2,3-dihydroxybutan-1,4-diyl)-2'-deoxyadenosine (N,N-DHB-dA). If not removed prior to DNA...
4.
Johnson L, Malayappan B, Tretyakova N, Campbell C, MacMillan M, Wagner J, et al.
Pediatr Blood Cancer . 2011 Jul; 58(5):708-14. PMID: 21793181
Background: Fanconi anemia (FA) patients are hypersensitive to DNA alkylating agents and require lower doses than non-FA patients to minimize serious toxicity. The mechanism by which hypersensitivity occurs is thought...
5.
Malayappan B, Johnson L, Nie B, Panchal D, Matter B, Jacobson P, et al.
Anal Chem . 2010 Apr; 82(9):3650-8. PMID: 20361772
Cyclophosphamide (CPA) is a DNA alkylating agent widely used in cancer chemotherapy. CPA undergoes metabolic activation to phosphoramide mustard and nornitrogen mustard (NOR) which alkylate the N-7 position of guanine...
6.
Hofer T, Fontana L, Anton S, Weiss E, Villareal D, Malayappan B, et al.
Rejuvenation Res . 2008 Aug; 11(4):793-9. PMID: 18729811
Excessive adiposity is associated with increased oxidative stress and accelerated aging. Weight loss induced by negative energy balance reduces markers of oxidation in experimental animals and humans. The long-term effects...
7.
Malayappan B, Garrett T, Segal M, Leeuwenburgh C
J Chromatogr A . 2007 Sep; 1167(1):54-62. PMID: 17765254
A sensitive and specific assay aimed at measuring the oxidized nucleic acids, 8-oxoguanine (8-oxoGua), fapy-guanine (Fapy-Gua), 8-oxoguanosine (8-oxoGuo), 8-oxo-2'-deoxyguanosine (8-oxodG) has been developed by coupling reversed phase liquid chromatography (HPLC)...