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Bart Leemans

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Recent Articles
11.
Leemans B, Stout T, De Schauwer C, Heras S, Nelis H, Hoogewijs M, et al.
Reproduction . 2019 Feb; 157(5):R181-R197. PMID: 30721132
In contrast to various other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. In particular, stallion spermatozoa fails to penetrate the zona pellucida, most...
12.
Pavani K, Hendrix A, Van Den Broeck W, Couck L, Szymanska K, Lin X, et al.
Int J Mol Sci . 2018 Dec; 20(1). PMID: 30577682
Extracellular vesicles (EVs) play a possible role in cell⁻cell communication and are found in various body fluids and cell conditioned culture media. The aim of this study was to isolate...
13.
Leemans B, Gadella B, Stout T, De Schauwer C, Nelis H, Hoogewijs M, et al.
Reproduction . 2016 Sep; 152(6):R233-R245. PMID: 27651517
In contrast to man and many other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. The apparent inability of stallion spermatozoa to penetrate the...
14.
Smits K, Nelis H, Van Steendam K, Govaere J, Roels K, Ververs C, et al.
Reprod Fertil Dev . 2016 Apr; 29(6):1085-1095. PMID: 27120206
The equine oviduct plays a pivotal role in providing the optimal microenvironment for early embryonic development, but little is known about the protein composition of the oviducal fluid in the...
15.
Leemans B, Gadella B, Stout T, Sostaric E, De Schauwer C, Nelis H, et al.
Reproduction . 2016 Jan; 151(4):313-30. PMID: 26755687
In many species, sperm binding to oviduct epithelium is believed to be an essential step in generating a highly fertile capacitated sperm population primed for fertilization. In several mammalian species,...
16.
Leemans B, Gadella B, Stout T, Nelis H, Hoogewijs M, Van Soom A
Reproduction . 2015 Aug; 150(3):193-208. PMID: 26242588
Induction of hyperactivated motility is considered essential for triggering the release of oviduct-bound mammalian spermatozoa in preparation for fertilization. In this study, oviduct-bound stallion spermatozoa were exposed for 2 h...
17.
Leemans B, Gadella B, Stout T, Heras S, Smits K, Ferrer-Buitrago M, et al.
Biol Reprod . 2015 Jun; 93(1):23. PMID: 26085521
Coincubating equine gametes in the presence of procaine has been reported to facilitate in vitro fertilization, with cleavage rates exceeding 60%. We report that while procaine does trigger sperm hyperactivation,...
18.
Nelis H, Wojciechowicz B, Franczak A, Leemans B, DHerde K, Goossens K, et al.
Reprod Fertil Dev . 2015 Jun; 28(12):1926-1944. PMID: 26085435
The oviduct undergoes dramatic functional and morphological changes throughout the oestrous cycle of the mare. To unravel the effects of steroids on the morphology, functionality and gene expression of the...
19.
Nelis H, Bussche J, Wojciechowicz B, Franczak A, Vanhaecke L, Leemans B, et al.
Reprod Fertil Dev . 2015 Mar; PMID: 25751414
Steroids play an important role in mammalian reproduction and early pregnancy. Although systemic changes in steroid concentrations have been well documented, it is not clear how these correlate with local...
20.
Heras S, Smits K, Leemans B, Van Soom A
Anal Biochem . 2014 Dec; 471:67-9. PMID: 25461478
Hoechst staining has traditionally been used to evaluate fertilization and parental origin of pronuclei. However, prevalence of parthenogenetic activation cannot be distinguished accurately by this protocol, and variation of relative...