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A A De Graaf

Explore the profile of A A De Graaf including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 39
Citations 1114
Followers 0
Related Specialties
Biochemistry
Biomedical Engineering
Microbiology
Top 10 Co-Authors
H Sahm
W Wiechert
W M Bovee
L Eggeling
M Mollney
N E Deutz
A Marx
V F Wendisch
B J Eikmanns
R A Chamuleau
Published In
Biotechnol Bioeng
Metab Eng
Adv Biochem Eng Biotechnol
Arch Microbiol
Magn Reson Med
Affiliations
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Recent Articles
11.
Pathway analysis and metabolic engineering in Corynebacterium glutamicum
Sahm H, Eggeling L, De Graaf A
Biol Chem . 2000 Nov; 381(9-10):899-910. PMID: 11076021
The gram-positive bacterium Corynebacterium glutamicum is used for the industrial production of amino acids, e.g. of L-glutamate and L-lysine. During the last 15 years, genetic engineering and amplification of genes...
12.
In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum
Petersen S, De Graaf A, Eggeling L, Mollney M, Wiechert W, Sahm H
J Biol Chem . 2000 Aug; 275(46):35932-41. PMID: 10946002
The C(3)-C(4) metabolite interconversion at the anaplerotic node in many microorganisms involves a complex set of reactions. C(3) carboxylation to oxaloacetate can originate from phosphoenolpyruvate and pyruvate, and at the...
13.
Response of the central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase
Marx A, Eikmanns B, Sahm H, De Graaf A, Eggeling L
Metab Eng . 2000 Aug; 1(1):35-48. PMID: 10935753
The extensive use of 13C enrichments in precursor metabolites for flux quantification does not rely on NADPH stoichiometries and can therefore be used to quantify reducing power fluxes. As an...
14.
Kinetic properties of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Corynebacterium glutamicum and their application for predicting pentose phosphate pathway flux in vivo
Moritz B, Striegel K, De Graaf A, Sahm H
Eur J Biochem . 2000 Jun; 267(12):3442-52. PMID: 10848959
The glucose-6-phosphate (Glc6P) and 6-phosphogluconate (6PG) dehydrogenases of the amino-acid-producing bacterium Corynebacterium glutamicum were purified to homogeneity and kinetically characterized. The Glc6P dehydrogenase was a heteromultimeric complex, which consists of...
15.
Quantitative determination of metabolic fluxes during coutilization of two carbon sources: comparative analyses with Corynebacterium glutamicum during growth on acetate and/or glucose
Wendisch V, De Graaf A, Sahm H, Eikmanns B
J Bacteriol . 2000 May; 182(11):3088-96. PMID: 10809686
Growth of Corynebacterium glutamicum on mixtures of the carbon sources glucose and acetate is shown to be distinct from growth on either substrate alone. The organism showed nondiauxic growth on...
16.
Determination of full 13C isotopomer distributions for metabolic flux analysis using heteronuclear spin echo difference NMR spectroscopy
De Graaf A, Mahle M, Mollney M, Wiechert W, Stahmann P, Sahm H
J Biotechnol . 2000 Feb; 77(1):25-35. PMID: 10674212
13C-isotopomer labeling experiments play an increasingly important role in the analysis of intracellular metabolic fluxes for genetic engineering purposes. 13C NMR spectroscopy is a key technique in the experimental determination...
17.
Bidirectional reaction steps in metabolic networks: IV. Optimal design of isotopomer labeling experiments
Mollney M, Wiechert W, Kownatzki D, De Graaf A
Biotechnol Bioeng . 1999 Nov; 66(2):86-103. PMID: 10567067
This article generalizes the statistical tools for the evaluation of carbon-labeling experiments that have been developed for the case of positional enrichment systems in part II of this series to...
18.
Bidirectional reaction steps in metabolic networks: III. Explicit solution and analysis of isotopomer labeling systems
Wiechert W, Mollney M, Isermann N, Wurzel M, De Graaf A
Biotechnol Bioeng . 1999 Nov; 66(2):69-85. PMID: 10567066
The last few years have brought tremendous progress in experimental methods for metabolic flux determination by carbon-labeling experiments. A significant enlargement of the available measurement data set has been achieved,...
19.
Metabolic state of Zymomonas mobilis in glucose-, fructose-, and xylose-fed continuous cultures as analysed by 13C- and 31P-NMR spectroscopy
De Graaf A, Striegel K, Wittig R, Laufer B, Schmitz G, Wiechert W, et al.
Arch Microbiol . 1999 Jun; 171(6):371-85. PMID: 10369893
The reasons for the well-known significantly different behaviour of the anaerobic, gram-negative, ethanologenic bacterium Zymomonas mobilis during growth on fructose (i.e. decreased growth and ethanol yields, increased by-product formation) as...
20.
Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol
Sprenger G, Schorken U, Wiegert T, Grolle S, De Graaf A, Taylor S, et al.
Proc Natl Acad Sci U S A . 1997 Dec; 94(24):12857-62. PMID: 9371765
In Escherichia coli, 1-deoxy-D-xylulose (or its 5-phosphate, DXP) is the biosynthetic precursor to isopentenyl diphosphate [Broers, S. T. J. (1994) Dissertation (Eidgenössische Technische Hochschule, Zürich)], thiamin, and pyridoxol [Himmeldirk, K.,...