Heterogeneity of Neural Progenitor Cells Revealed by Enhancers in the Nestin Gene

Overview

Journal Dev Biol

Publisher Elsevier

Specialties Biology
Reproductive Medicine

Date 1999 Jan 26

PMID 9917366

Citations 41

Authors

P J Yaworsky

C Kappen

Affiliations

Soon will be listed here.

Abstract

Using transgenic embryos, we have identified two distinct CNS progenitor cell-specific enhancers, each requiring the cooperation of at least two independent regulatory sites, within the second intron of the rat nestin gene. One enhancer is active throughout the developing CNS, while the other is specifically active in the ventral midbrain. These experiments demonstrate that neural progenitor cells in the midbrain constitute a unique subpopulation based upon their ability to activate the midbrain regulatory element. Our finding of differential enhancer activity from a gene encoding a structural protein reveals a previously unrecognized diversity in neural progenitor cell populations.

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References

Marks M, Hallenbeck P, Nagata T, Segars J, Appella E, Nikodem V . H-2RIIBP (RXR beta) heterodimerization provides a mechanism for combinatorial diversity in the regulation of retinoic acid and thyroid hormone responsive genes. EMBO J. 1992; 11(4):1419-35. PMC: 556591. DOI: 10.1002/j.1460-2075.1992.tb05187.x. View

Dahlstrand J, Collins V, Lendahl U . Expression of the class VI intermediate filament nestin in human central nervous system tumors. Cancer Res. 1992; 52(19):5334-41. View

Stemple D, Anderson D . Isolation of a stem cell for neurons and glia from the mammalian neural crest. Cell. 1992; 71(6):973-85. DOI: 10.1016/0092-8674(92)90393-q. View

Dahlstrand J, Zimmerman L, McKay R, Lendahl U . Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments. J Cell Sci. 1992; 103 ( Pt 2):589-97. DOI: 10.1242/jcs.103.2.589. View

Law S, Conneely O, Demayo F, OMalley B . Identification of a new brain-specific transcription factor, NURR1. Mol Endocrinol. 1992; 6(12):2129-35. DOI: 10.1210/mend.6.12.1491694. View

Tohyama T, Lee V, RORKE L, Marvin M, McKay R, Trojanowski J . Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells. Lab Invest. 1992; 66(3):303-13. View

Reynolds B, Weiss S . Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 1992; 255(5052):1707-10. DOI: 10.1126/science.1553558. View

Lendahl U, Zimmerman L, McKay R . CNS stem cells express a new class of intermediate filament protein. Cell. 1990; 60(4):585-95. DOI: 10.1016/0092-8674(90)90662-x. View

Renfranz P, Cunningham M, McKay R . Region-specific differentiation of the hippocampal stem cell line HiB5 upon implantation into the developing mammalian brain. Cell. 1991; 66(4):713-29. DOI: 10.1016/0092-8674(91)90116-g. View

10.

Peers B, Voz M, Monget P, Mathy-Hartert M, Berwaer M, Belayew A . Regulatory elements controlling pituitary-specific expression of the human prolactin gene. Mol Cell Biol. 1990; 10(9):4690-700. PMC: 361059. DOI: 10.1128/mcb.10.9.4690-4700.1990. View

11.

Wetts R, Fraser S . Multipotent precursors can give rise to all major cell types of the frog retina. Science. 1988; 239(4844):1142-5. DOI: 10.1126/science.2449732. View

12.

He X, Treacy M, SIMMONS D, Ingraham H, Swanson L, Rosenfeld M . Expression of a large family of POU-domain regulatory genes in mammalian brain development. Nature. 1989; 340(6228):35-41. DOI: 10.1038/340035a0. View

13.

Frederiksen K, McKay R . Proliferation and differentiation of rat neuroepithelial precursor cells in vivo. J Neurosci. 1988; 8(4):1144-51. PMC: 6569254. View

14.

Turner D, Cepko C . A common progenitor for neurons and glia persists in rat retina late in development. Nature. 1987; 328(6126):131-6. DOI: 10.1038/328131a0. View

15.

Hockfield S, McKay R . Identification of major cell classes in the developing mammalian nervous system. J Neurosci. 1985; 5(12):3310-28. PMC: 6565218. View

16.

Vicario-Abejon C, Cunningham M, McKay R . Cerebellar precursors transplanted to the neonatal dentate gyrus express features characteristic of hippocampal neurons. J Neurosci. 1995; 15(10):6351-63. PMC: 6578006. View

17.

Bayer S, Wills K, Triarhou L, Ghetti B . Time of neuron origin and gradients of neurogenesis in midbrain dopaminergic neurons in the mouse. Exp Brain Res. 1995; 105(2):191-9. DOI: 10.1007/BF00240955. View

18.

Kachinsky A, Dominov J, Miller J . Intermediate filaments in cardiac myogenesis: nestin in the developing mouse heart. J Histochem Cytochem. 1995; 43(8):843-7. DOI: 10.1177/43.8.7542682. View

19.

Frisen J, Johansson C, Torok C, Risling M, Lendahl U . Rapid, widespread, and longlasting induction of nestin contributes to the generation of glial scar tissue after CNS injury. J Cell Biol. 1995; 131(2):453-64. PMC: 2199991. DOI: 10.1083/jcb.131.2.453. View

20.

Williams B, Price J . Evidence for multiple precursor cell types in the embryonic rat cerebral cortex. Neuron. 1995; 14(6):1181-8. DOI: 10.1016/0896-6273(95)90265-1. View