Evidence for Suppressor Cell Activity Associated with Depression of Contact Sensitivity in Pseudomonas Aeruginosa Infected Mice

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1976 Nov 1

PMID 991463

Citations 6

Authors

M Campa

C Garzelli

E Ferrannini

G Falcone

Affiliations

Soon will be listed here.

Abstract

Pseudomonas aeruginosa infection depresses contact sensitivity to 2-phenyl-4-ethoximethylene-oxazolone (oxazolone), and enhances the antibody response to sheep erythrocytes (SRBC) in the mouse. Anti-oxazolone antibody titres were found not to be significantly different in infected and uninfected animals; thus, the major circulating classes of antibodies do not seem to be responsibile for the observed depression of skin reactivity. Low dose (20 mg/Kg) cyclophosphamide (CY) pretreatment induced a further potentiation of antibody response to SRBC, and prevented depression of contact sensitivity in infected mice. On the other hand, when infected animals were pretreated with high doses (200 mg/Kg) of CY, antibody production was completely suppressed, whereas contact sensitivity was unaffected. Since CY treatment is known to selectively inhibit B lymphocytes, and since it can abrogate the infection-induced depression of reactivity to oxazolone, it is suggested that suppressor cells, which may have B-cell characteristics, are stimulated during P. aeruginosa infection in the mouse.

Citing Articles

Cell-mediated immunity and delayed-type hypersensitivity in Pseudomonas aeruginosa-infected mice.

Campa M, Toca L, Lombardi S, Garzelli C, Colizzi V, Falcone G Med Microbiol Immunol. 1982; 170(3):191-9.

PMID: 7078523 DOI: 10.1007/BF02298199.

Mouse footpad infection by Pseudomonas aeruginosa: evidence for delayed hypersensitivity to specific bacterial antigen.

Garzelli C, Colizzi V, Campa M, Falcone G Med Microbiol Immunol. 1980; 168(2):111-8.

PMID: 6779101 DOI: 10.1007/BF02121759.

Alteration of murine immune response by Pseudomonas aeruginosa exotoxin A.

Holt P, Misfeldt M Infect Immun. 1984; 45(1):227-33.

PMID: 6429047 PMC: 263305. DOI: 10.1128/iai.45.1.227-233.1984.

Depression of contact sensitivity by Pseudomonas aeruginosa-induced suppressor cells which affect the induction phase of immune response.

Garzelli C, Colizzi V, Campa M, Bozzi L, Falcone G Infect Immun. 1979; 26(1):4-11.

PMID: 159263 PMC: 414565. DOI: 10.1128/iai.26.1.4-11.1979.

Pseudomonas aeruginosa infection depresses contact sensitivity to oxazolone by enhancing suppressor cell activity.

Colizzi V, Campa M, Garzelli C, Falcone G Med Microbiol Immunol. 1979; 167(3):181-8.

PMID: 158702 DOI: 10.1007/BF02121184.

References

Zembala M, Asherson G . The effect of cyclophosphamide and irradiation on cells which suppress contact sensitivity in the mouse. Clin Exp Immunol. 1976; 23(3):554-61. PMC: 1538389. View

Bendinelli M, Toniolo A, Campa M . Immunodepression by Rowson-Parr virus in mice: effect of Rowson-Parr virus and Friend leukemia complex infections on background antibody-forming cells to various erythrocytes. Infect Immun. 1975; 11(5):1024-30. PMC: 415175. DOI: 10.1128/iai.11.5.1024-1030.1975. View

Askenase P, Hayden B, GERSHON R . Augmentation of delayed-type hypersensitivity by doses of cyclophosphamide which do not affect antibody responses. J Exp Med. 1975; 141(3):697-702. PMC: 2189708. DOI: 10.1084/jem.141.3.697. View

Askenase P, Asherson G . Contact sensitivity to oxazolone in the mouse. VIII. Demonstration of several classes of antibody in the sera of contact sensitized and unimmunized mice by a simplified antiglobulin assay. Immunology. 1972; 23(3):289-98. PMC: 1407925. View

Sensakovic J, Bartell P . The slime of Pseudomonas aeruginosa: biological characterization and possible role in experimental infection. J Infect Dis. 1974; 129(2):101-9. DOI: 10.1093/infdis/129.2.101. View

Polak L, Turk J . Reversal of immunological tolerance by cyclophosphamide through inhibition of suppressor cell activity. Nature. 1974; 249(458):654-6. DOI: 10.1038/249654a0. View

Scott M . Depression of delayed-type hypersensitivity by Corynebacterium parvum: mandatory role of the spleen. Cell Immunol. 1974; 13(2):251-63. DOI: 10.1016/0008-8749(74)90243-3. View

Turk J, Parker D, Poulter L . Functional aspects of the selective depletion of lymphoid tissue by cyclophosphamide. Immunology. 1972; 23(4):493-501. PMC: 1407968. View

Turk J, Parker D . Further studies on B-lymphocyte suppression in delayed hypersensitivity, indicating a possible mechanism for Jones-Mote hypersensitivity. Immunology. 1973; 24(4):751-8. PMC: 1422890. View

10.

Phanupak P, Moorhead J, Claman H . Tolerance and contact sensitivity to DNFB in mice. 3. Transfer of tolerance with "suppressor T cells". J Immunol. 1974; 113(4):1230-6. View

11.

Zembala M, Asherson G . Depression of the T cell phenomenon of contact sensitivity by T cells from unresponsive mice. Nature. 1973; 244(5413):227-8. DOI: 10.1038/244227a0. View

12.

Asherson G, Barnes R . Contact sensitivity in the mouse. IX. The role of immunological and non-immunological inflammation in the movement of lymphocytes to immunized lymph nodes. Immunology. 1973; 24(5):885-94. PMC: 1422815. View

13.

Katz S, Parker D, Sommer G, Turk J . Suppressor cells in normal immunisation as a basic homeostatic phenomenon. Nature. 1974; 248(449):612-4. DOI: 10.1038/248612a0. View

14.

UHR J, Moller G . Regulatory effect of antibody on the immune response. Adv Immunol. 1968; 8:81-127. DOI: 10.1016/s0065-2776(08)60465-4. View

15.

DE SOUSA M, Parrott D . Induction and recall in contact sensivitity. Changes in skin and draining lymph nodes of intact and thymectomized mice. J Exp Med. 1969; 130(4):671-90. PMC: 2138717. DOI: 10.1084/jem.130.4.671. View

16.

Davies A, Carter R, LEUCHARS E, Wallis V . The morphology of immune reactions in normal, thymectomized and reconstituted mice. II. The response to oxazolone. Immunology. 1969; 17(1):111-26. PMC: 1455937. View

17.

STANIER R, Palleroni N, DOUDOROFF M . The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966; 43(2):159-271. DOI: 10.1099/00221287-43-2-159. View

18.

de Hurtado I, OVARY Z, OSLER A . The humoral response to DNCB: its detection by different techniques. Proc Soc Exp Biol Med. 1975; 149(3):604-9. DOI: 10.3181/00379727-149-38861. View

19.

Campa M, Garzelli C, Falcone G . Depression of contact sensitivity and enhancement of antibody response in Pseudomonas aeruginosa-infected mice. Infect Immun. 1975; 12(6):1252-7. PMC: 415428. DOI: 10.1128/iai.12.6.1252-1257.1975. View