Cell-mediated Immunity and Delayed-type Hypersensitivity in Pseudomonas Aeruginosa-infected Mice

Overview

Journal Med Microbiol Immunol

Specialty Allergy & Immunology

Date 1982 Jan 1

PMID 7078523

Citations 2

Authors

M Campa

L Toca

S Lombardi

C Garzelli

V Colizzi

G Falcone

Affiliations

Soon will be listed here.

Abstract

In mice repeated systemic injections of Pseudomonas aeruginosa viable cells were able to induce a specific delayed-type hypersensitivity, which was evaluated as increase both in footpad swelling and in the weight of popliteal lymph nodes, after a challenge in the footpad. Unfractionated spleen cells or T lymphocyte-enriched spleen cells from sensitized donors were able to specifically transfer the delayed-type hypersensitivity to syngeneic recipients but failed to protect them against a lethal challenge with P. aeruginosa. In contrast, serum or B lymphocyte and macrophage-enriched spleen cells from the same donors were capable of transferring protective immunity but failed to induce any delayed-type hypersensitivity reaction in the recipients. These results clearly show that in systemic P. aeruginosa infections a dissociation between delayed-type hypersensitivity and acquired cellular resistance occurs.

Citing Articles

Impairment of cell-mediated immunity in Pseudomonas aeruginosa pyelonephritis: lack of suppressor cell activity in vivo.

Colizzi V, Campa M, Garzelli C, Toca L, Bevilacqua G, Falcone G Med Microbiol Immunol. 1982; 171(3):123-33.

PMID: 6219276 DOI: 10.1007/BF02123620.

Mapping of the T-cell recognition sites of Pseudomonas aeruginosa PAK polar pili.

Smart W, Sastry P, Paranchych W, Singh B Infect Immun. 1988; 56(1):18-23.

PMID: 2447019 PMC: 259226. DOI: 10.1128/iai.56.1.18-23.1988.

References

Reynolds H . Pulmonary host defenses in rabbits after immunization with Pseudomonas antigens: the interaction of bacteria, antibodies, macrophages, and lymphocytes. J Infect Dis. 1974; 130 Suppl(0):S134-42. DOI: 10.1093/infdis/130.supplement.s134. View

Pierson C, Johnson A, Feller I . Effect of cyclophosphamide on the immune response to Pseudomonas aeruginosa in mice. Infect Immun. 1976; 14(1):168-77. PMC: 420860. DOI: 10.1128/iai.14.1.168-177.1976. View

Mackaness G . THE IMMUNOLOGICAL BASIS OF ACQUIRED CELLULAR RESISTANCE. J Exp Med. 1964; 120:105-20. PMC: 2137723. DOI: 10.1084/jem.120.1.105. View

Munster A, Leary A . Cell-mediated immune responses to Pseudomonas aeruginosa. Am J Surg. 1977; 133(6):710-2. DOI: 10.1016/0002-9610(77)90160-x. View

SALAMAN M, Wedderburn N, Festenstein H, Huber B . Detection of a graft-versus-host reaction between mice compatible at the H-2 locus. Transplantation. 1973; 16(1):29-31. DOI: 10.1097/00007890-197307000-00006. View

Tribble J, Bolen J . Induction and specificity of delayed hypersensitivity to Staphylococcus aureus in mice. Immunology. 1978; 35(1):21-32. PMC: 1457225. View

Garzelli C, Colizzi V, Campa M, Falcone G . Mouse footpad infection by Pseudomonas aeruginosa: evidence for delayed hypersensitivity to specific bacterial antigen. Med Microbiol Immunol. 1980; 168(2):111-8. DOI: 10.1007/BF02121759. View

Mackaness G . The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969; 129(5):973-92. PMC: 2138649. DOI: 10.1084/jem.129.5.973. View

Neiburger R, YOUMANS G, YOUMANS A . Relationship between tuberculin hypersensitivity and cellular immunity to infection in mice vaccinated with viable attenuated Mycobacterial cells or with Mycobacterial ribonucleic acid preparations. Infect Immun. 1973; 8(1):42-7. PMC: 422807. DOI: 10.1128/iai.8.1.42-47.1973. View

10.

Garzelli C, Colizzi V, Campa M, Bozzi L, Falcone G . Depression of contact sensitivity by Pseudomonas aeruginosa-induced suppressor cells which affect the induction phase of immune response. Infect Immun. 1979; 26(1):4-11. PMC: 414565. DOI: 10.1128/iai.26.1.4-11.1979. View

11.

Dustoor M, Blazkovec A . Acquired cellular resistance, delayed hypersensitivity, and altered macrophage migration in Listeria monocytogenes-infected guinea pigs. Infect Immun. 1978; 21(1):10-6. PMC: 421950. DOI: 10.1128/iai.21.1.10-16.1978. View

12.

Escande A, Cannat A, Serre A . Dissociation of delayed-type hypersensitivity from acquired murine resistance to Brucella suis. Ann Immunol (Paris). 1980; 131D(2):199-204. View

13.

Easmon C, GLYNN A . Cell-mediated immune responses in Staphylococcus aureus infections in mice. Immunology. 1975; 29(1):75-85. PMC: 1445861. View

14.

Taubler J, MUDD S . Staphylococcal delayed hypersensitivity in mice. II. In vitro demonstration and specificity of delayed hypersensitivity. J Immunol. 1968; 101(3):550-5. View

15.

Sandok P, HINSDILL R, Albrecht R . Migration inhibition of mouse macrophages by Brucella antigens. Infect Immun. 1971; 4(4):516-8. PMC: 416343. DOI: 10.1128/iai.4.4.516-518.1971. View

16.

Collins F, Mackaness G . Delayed hypersensitivity and arthus reactivity in relation to host resistance in salmonella-infected mice. J Immunol. 1968; 101(5):830-45. View

17.

FISHMAN L, Armstrong D . Pseudomonas aeruginosa bacteremia in patients with neoplastic disease. Cancer. 1972; 30(3):764-73. DOI: 10.1002/1097-0142(197209)30:3<764::aid-cncr2820300326>3.0.co;2-g. View

18.

Young L . Human immunity to Pseudomonas aeruginosa. II. Relationship between heat-stable opsonins and type-specific lipopolysaccharides. J Infect Dis. 1972; 126(3):277-87. DOI: 10.1093/infdis/126.3.277. View

19.

Hurtrel B, Langrange P, MICHEL J . Absence of correlation between delayed-type hypersensitivity and protection in experimental systemic candidiasis in immunized mice. Infect Immun. 1981; 31(1):95-101. PMC: 351756. DOI: 10.1128/iai.31.1.95-101.1981. View

20.

Trizio D, CUDKOWICZ G . Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo. J Immunol. 1974; 113(4):1093-7. View