Gram-negative Bacteria Produce Membrane Vesicles Which Are Capable of Killing Other Bacteria

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1998 Oct 10

PMID 9765585

Citations 117

Authors

Z Li

A J Clarke

T J Beveridge

Affiliations

Soon will be listed here.

Abstract

Naturally produced membrane vesicles (MVs), isolated from 15 strains of gram-negative bacteria (Citrobacter, Enterobacter, Escherichia, Klebsiella, Morganella, Proteus, Salmonella, and Shigella strains), lysed many gram-positive (including Mycobacterium) and gram-negative cultures. Peptidoglycan zymograms suggested that MVs contained peptidoglycan hydrolases, and electron microscopy revealed that the murein sacculi were digested, confirming a previous modus operandi (J. L. Kadurugamuwa and T. J. Beveridge, J. Bacteriol. 174:2767-2774, 1996). MV-sensitive bacteria possessed A1alpha, A4alpha, A1gamma, A2alpha, and A4gamma peptidoglycan chemotypes, whereas A3alpha, A3beta, A3gamma, A4beta, B1alpha, and B1beta chemotypes were not affected. Pseudomonas aeruginosa PAO1 vesicles possessed the most lytic activity.

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References

Chatterjee S, Das J . Electron microscopic observations on the excretion of cell-wall material by Vibrio cholerae. J Gen Microbiol. 1967; 49(1):1-11. DOI: 10.1099/00221287-49-1-1. View

Wispelwey B, Hansen E, Scheld W . Haemophilus influenzae outer membrane vesicle-induced blood-brain barrier permeability during experimental meningitis. Infect Immun. 1989; 57(8):2559-62. PMC: 313486. DOI: 10.1128/iai.57.8.2559-2562.1989. View

DeVoe I, Gilchrist J . Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis. J Exp Med. 1973; 138(5):1156-67. PMC: 2139435. DOI: 10.1084/jem.138.5.1156. View

Holtje J, Tomasz A . Lipoteichoic acid: a specific inhibitor of autolysin activity in Pneumococcus. Proc Natl Acad Sci U S A. 1975; 72(5):1690-4. PMC: 432610. DOI: 10.1073/pnas.72.5.1690. View

Cleveland R, Holtje J, WICKEN A, Tomasz A, SHOCKMAN G . Inhibition of bacterial wall lysins by lipoteichoic acids and related compounds. Biochem Biophys Res Commun. 1975; 67(3):1128-35. DOI: 10.1016/0006-291x(75)90791-3. View

Cleveland R, WICKEN A, SHOCKMAN G . Effect of lipoteichoic acid and lipids on lysis of intact cells of Streptococcus faecalis. J Bacteriol. 1976; 127(3):1582-4. PMC: 232960. DOI: 10.1128/jb.127.3.1582-1584.1976. View

Dorward D, Garon C, JUDD R . Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae. J Bacteriol. 1989; 171(5):2499-505. PMC: 209926. DOI: 10.1128/jb.171.5.2499-2505.1989. View

Graham L, Harris R, Villiger W, Beveridge T . Freeze-substitution of gram-negative eubacteria: general cell morphology and envelope profiles. J Bacteriol. 1991; 173(5):1623-33. PMC: 207311. DOI: 10.1128/jb.173.5.1623-1633.1991. View

Paul T, Beveridge T . Reevaluation of envelope profiles and cytoplasmic ultrastructure of mycobacteria processed by conventional embedding and freeze-substitution protocols. J Bacteriol. 1992; 174(20):6508-17. PMC: 207613. DOI: 10.1128/jb.174.20.6508-6517.1992. View

10.

Whitmire W, Garon C . Specific and nonspecific responses of murine B cells to membrane blebs of Borrelia burgdorferi. Infect Immun. 1993; 61(4):1460-7. PMC: 281386. DOI: 10.1128/iai.61.4.1460-1467.1993. View

11.

Pugsley A . The complete general secretory pathway in gram-negative bacteria. Microbiol Rev. 1993; 57(1):50-108. PMC: 372901. DOI: 10.1128/mr.57.1.50-108.1993. View

12.

Kondo K, Takade A, Amako K . Release of the outer membrane vesicles from Vibrio cholerae and Vibrio parahaemolyticus. Microbiol Immunol. 1993; 37(2):149-52. DOI: 10.1111/j.1348-0421.1993.tb03192.x. View

13.

Paul T, Beveridge T . Preservation of surface lipids and determination of ultrastructure of Mycobacterium kansasii by freeze-substitution. Infect Immun. 1994; 62(5):1542-50. PMC: 186351. DOI: 10.1128/iai.62.5.1542-1550.1994. View

14.

Watt S, Clarke A . Initial characterization of two extracellular autolysins from Pseudomonas aeruginosa PAO1. J Bacteriol. 1994; 176(15):4784-9. PMC: 196306. DOI: 10.1128/jb.176.15.4784-4789.1994. View

15.

Bernadsky G, Beveridge T, Clarke A . Analysis of the sodium dodecyl sulfate-stable peptidoglycan autolysins of select gram-negative pathogens by using renaturing polyacrylamide gel electrophoresis. J Bacteriol. 1994; 176(17):5225-32. PMC: 196705. DOI: 10.1128/jb.176.17.5225-5232.1994. View

16.

Kadurugamuwa J, Beveridge T . Virulence factors are released from Pseudomonas aeruginosa in association with membrane vesicles during normal growth and exposure to gentamicin: a novel mechanism of enzyme secretion. J Bacteriol. 1995; 177(14):3998-4008. PMC: 177130. DOI: 10.1128/jb.177.14.3998-4008.1995. View

17.

Wai S, Takade A, Amako K . The release of outer membrane vesicles from the strains of enterotoxigenic Escherichia coli. Microbiol Immunol. 1995; 39(7):451-6. DOI: 10.1111/j.1348-0421.1995.tb02228.x. View

18.

Li Z, Clarke A, Beveridge T . A major autolysin of Pseudomonas aeruginosa: subcellular distribution, potential role in cell growth and division and secretion in surface membrane vesicles. J Bacteriol. 1996; 178(9):2479-88. PMC: 177969. DOI: 10.1128/jb.178.9.2479-2488.1996. View

19.

Kadurugamuwa J, Beveridge T . Bacteriolytic effect of membrane vesicles from Pseudomonas aeruginosa on other bacteria including pathogens: conceptually new antibiotics. J Bacteriol. 1996; 178(10):2767-74. PMC: 178010. DOI: 10.1128/jb.178.10.2767-2774.1996. View

20.

Beveridge T, Makin S, Kadurugamuwa J, Li Z . Interactions between biofilms and the environment. FEMS Microbiol Rev. 1997; 20(3-4):291-303. DOI: 10.1111/j.1574-6976.1997.tb00315.x. View