Use of Microsphere Technology for Targeted Delivery of Rifampin to Mycobacterium Tuberculosis-infected Macrophages

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1998 Oct 3

PMID 9756777

Citations 24

Authors

E L Barrow

G A Winchester

J K Staas

D C Quenelle

W W Barrow

Affiliations

Soon will be listed here.

Abstract

Microsphere technology was used to develop formulations of rifampin for targeted delivery to host macrophages. These formulations were prepared by using biocompatible polymeric excipients of lactide and glycolide copolymers. Release characteristics were examined in vitro and also in two monocytic cell lines, the murine J774 and the human Mono Mac 6 cell lines. Bioassay assessment of cell culture supernatants from monocyte cell lines showed release of bioactive rifampin during a 7-day experimental period. Treatment of Mycobacterium tuberculosis H37Rv-infected monocyte cell lines with rifampin-loaded microspheres resulted in a significant decrease in numbers of CFU at 7 days following initial infection, even though only 8% of the microsphere-loaded rifampin was released. The levels of rifampin released from microsphere formulations within monocytes were more effective at reducing M. tuberculosis intracellular growth than equivalent doses of rifampin given as a free drug. These results demonstrate that rifampin-loaded microspheres can be formulated for effective sustained and targeted delivery to host macrophages.

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References

Cowsar D, Tice T, Gilley R, ENGLISH J . Poly(lactide-co-glycolide) microcapsules for controlled release of steroids. Methods Enzymol. 1985; 112:101-16. DOI: 10.1016/s0076-6879(85)12010-0. View

Morrow P . Conference on the scientific basis of respiratory therapy. Aerosol therapy. Aerosol characterization and deposition. Am Rev Respir Dis. 1974; 110(6 Pt 2):88-99. DOI: 10.1164/arrd.1974.110.6P2.88. View

Vladimirsky M, Ladigina G . Antibacterial activity of liposome-entrapped streptomycin in mice infected with mycobacterium tuberculosis. Biomed Pharmacother. 1982; 36(8-9):375-7. View

Thiel E, Futterer A, Herzog V, Wirtz A, Riethmuller G . Establishment of a human cell line (Mono Mac 6) with characteristics of mature monocytes. Int J Cancer. 1988; 41(3):456-61. DOI: 10.1002/ijc.2910410324. View

Amin N . Let's stop the comeback of tuberculosis. Best drug regimens for prevention and treatment. Postgrad Med. 1990; 88(6):107-14, 117-9, 122-4. DOI: 10.1080/00325481.1990.11716425. View

Fine P . Leprosy and tuberculosis--an epidemiological comparison. Tubercle. 1984; 65(2):137-53. DOI: 10.1016/0041-3879(84)90067-9. View

Hora M, Rana R, Nunberg J, Tice T, Gilley R, Hudson M . Release of human serum albumin from poly(lactide-co-glycolide) microspheres. Pharm Res. 1990; 7(11):1190-4. DOI: 10.1023/a:1015948829632. View

Visscher G, Robison R, Maulding H, Fong J, Pearson J, Argentieri G . Biodegradation of and tissue reaction to poly(DL-lactide) microcapsules. J Biomed Mater Res. 1986; 20(5):667-76. DOI: 10.1002/jbm.820200510. View

. WHO declares tuberculosis a global emergency. Soz Praventivmed. 1993; 38(4):251-2. View

10.

Jacob E, Setterstrom J, Bach D, Heath 3rd J, McNiesh L, Cierny 3rd G . Evaluation of biodegradable ampicillin anhydrate microcapsules for local treatment of experimental staphylococcal osteomyelitis. Clin Orthop Relat Res. 1991; (267):237-44. View

11.

Ehlers S, Bucke W, Leitzke S, Fortmann L, Smith D, Hansch H . Liposomal amikacin for treatment of M. avium infections in clinically relevant experimental settings. Zentralbl Bakteriol. 1996; 284(2-3):218-31. DOI: 10.1016/s0934-8840(96)80097-1. View

12.

. Control of tuberculosis in the United States. American Thoracic Society. Am Rev Respir Dis. 1992; 146(6):1623-33. DOI: 10.1164/ajrccm/146.6.1623. View

13.

Nightingale S, Saletan S, Swenson C, Lawrence A, WATSON D, Pilkiewicz F . Liposome-encapsulated gentamicin treatment of Mycobacterium avium-Mycobacterium intracellulare complex bacteremia in AIDS patients. Antimicrob Agents Chemother. 1993; 37(9):1869-72. PMC: 188084. DOI: 10.1128/AAC.37.9.1869. View

14.

. Nosocomial transmission of multidrug-resistant tuberculosis to health-care workers and HIV-infected patients in an urban hospital--Florida. MMWR Morb Mortal Wkly Rep. 1990; 39(40):718-22. View

15.

Wright E, Quenelle D, Suling W, Barrow W . Use of Mono Mac 6 human monocytic cell line and J774 murine macrophage cell line in parallel antimycobacterial drug studies. Antimicrob Agents Chemother. 1996; 40(9):2206-8. PMC: 163501. DOI: 10.1128/AAC.40.9.2206. View

16.

Bermudez L, Wu M, Young L . Intracellular killing of Mycobacterium avium complex by rifapentine and liposome-encapsulated amikacin. J Infect Dis. 1987; 156(3):510-3. DOI: 10.1093/infdis/156.3.510. View

17.

GANGADHARAM P, Ashtekar D, Flasher D, Duzgunes N . Therapy of Mycobacterium avium complex infections in beige mice with streptomycin encapsulated in sterically stabilized liposomes. Antimicrob Agents Chemother. 1995; 39(3):725-30. PMC: 162612. DOI: 10.1128/AAC.39.3.725. View

18.

Barksdale L, Kim K . Mycobacterium. Bacteriol Rev. 1977; 41(1):217-372. PMC: 414000. DOI: 10.1128/br.41.1.217-372.1977. View

19.

Visscher G, Robison M, Argentieri G . Tissue response to biodegradable injectable microcapsules. J Biomater Appl. 1987; 2(1):118-31. DOI: 10.1177/088532828700200103. View

20.

Visscher G, Robison R, Maulding H, Fong J, Pearson J, Argentieri G . Biodegradation of and tissue reaction to 50:50 poly(DL-lactide-co-glycolide) microcapsules. J Biomed Mater Res. 1985; 19(3):349-65. DOI: 10.1002/jbm.820190315. View