The Absence of Histone H1 from the Chromatin Fraction Obtained by Sonication of Calf Thymus Nuclei Under "quasiphysiological" Ionic Conditions

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1976 Aug 1

PMID 967688

Authors

A I Lishanskaya

M I Mosevitsky

Affiliations

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Abstract

The minor chromatin fraction was isolated from the sonicated calf thymus nuclei on the basis of its differential solubility in the "quasiphysiological" salt medium (0.1 M KCl-0.05 M NaCl-l mM MgCl2-1 mM CaCl2). Histone Hl is almost completely absent from this fraction. DNA isolated from this fraction occurs in three discrete low mol. wt. fragments. The fraction of chromatin which lacks histone Hl can also be obtained by two other methods. On of them consists in salt precipitation of the chromatin gel and its subsequent sonication. The second method includes precipitation of the sonicated chromatin gel by salts. In the first case the properties of the chromatin fraction which remains in the supernatant after centrifugation closely resemble those of the original salt-soluble nuclear fraction. The second method yields supernatant fraction also lacking histone Hl but containing heterogeneous DNA. Comparisons were also made of the sonically-solubilized nuclear fractions obtained in the complete salt medium and its mono and divalent cationic constituents.

References

FRENSTER J, Allfrey V, Mirsky A . REPRESSED AND ACTIVE CHROMATIN ISOLATED FROM INTERPHASE LYMPHOCYTES. Proc Natl Acad Sci U S A. 1963; 50:1026-32. PMC: 221266. DOI: 10.1073/pnas.50.6.1026. View

Littau V, Burdick C, Allfrey V, Mirsky S . The role of histones in the maintenance of chromatin structure. Proc Natl Acad Sci U S A. 1965; 54(4):1204-12. PMC: 219838. DOI: 10.1073/pnas.54.4.1204. View

Gottesfeld J, Garrard W, Bagi G, Wilson R, Bonner J . Partial purification of the template-active fraction of chromatin: a preliminary report. Proc Natl Acad Sci U S A. 1974; 71(6):2193-7. PMC: 388417. DOI: 10.1073/pnas.71.6.2193. View

Reeck G, Simpson R, SOBER H . Resolution of a spectrum of nucleoprotein species in sonicated chromatin. Proc Natl Acad Sci U S A. 1972; 69(8):2317-21. PMC: 426925. DOI: 10.1073/pnas.69.8.2317. View

Bonner J, Gottesfeld J, GARRARD W, Billing R, Uphouse L . Isolation of template active and inactive regions of chromatin. Methods Enzymol. 1975; 40:97-102. DOI: 10.1016/s0076-6879(75)40010-6. View

Reeck G, Simpson R, SOBER H . The distribution of histones and nonhistone proteins in the ECTHAM-cellulose fractions of chromatin from several tissues. Eur J Biochem. 1974; 49(2):407-14. DOI: 10.1111/j.1432-1033.1974.tb03846.x. View

Warnecke P, Kruse K, HARBERS E . Studies on deoxyribonucleoproteins. 8. Isolation and characterization of nonhistone proteins from euchromatic and heterochromatic deoxyribonucleoprotein of rat liver. Biochim Biophys Acta. 1973; 331(2):295-304. View

MCCONAUGHY B, McCarthy B . Fractionation of chromatin by thermal chromatography. Biochemistry. 1972; 11(6):998-1003. DOI: 10.1021/bi00756a008. View

Marushige K, Bonner J . Fractionation of liver chromatin. Proc Natl Acad Sci U S A. 1971; 68(12):2941-4. PMC: 389565. DOI: 10.1073/pnas.68.12.2941. View

10.

Berkowitz E, Doty P . Chemical and physical properties of fractionated chromatin. Proc Natl Acad Sci U S A. 1975; 72(9):3328-32. PMC: 432985. DOI: 10.1073/pnas.72.9.3328. View

11.

Gottesfeld J, Murphy R, Bonner J . Structure of transcriptionally active chromatin. Proc Natl Acad Sci U S A. 1975; 72(11):4404-8. PMC: 388730. DOI: 10.1073/pnas.72.11.4404. View

12.

Varshavsky A, Bakayev V . Studies on chromatin. III. v-Bodies and free DNA in chromatin lacking histone H1. Mol Biol Rep. 1975; 2(3):209-17. DOI: 10.1007/BF00356990. View

13.

Oudet P, Chambon P . Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975; 4(4):281-300. DOI: 10.1016/0092-8674(75)90149-x. View

14.

Lishanskaya A, Mosevitsky M . Study of calf thymus deoxyribonucleoproteins by means of gel electrophoresis. Effect of ionic composition on the mode of chromatin fragmentation. Biochem Biophys Res Commun. 1975; 62(4):822-9. DOI: 10.1016/0006-291x(75)90396-4. View

15.

Yasmineh W, Yunis J . Satellite DNA in mouse autosomal heterochromatin. Biochem Biophys Res Commun. 1969; 35(6):779-82. DOI: 10.1016/0006-291x(69)90690-1. View

16.

Panyim S, Chalkley R . High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969; 130(1):337-46. DOI: 10.1016/0003-9861(69)90042-3. View

17.

Chalkley R, Jensen R . A study of the structure of isolated chromatin. Biochemistry. 1968; 7(12):4380-8. DOI: 10.1021/bi00852a034. View

18.

Samarina O, Lukanidin E, Molnar J, Georgiev G . Structural organization of nuclear complexes containing DNA-like RNA. J Mol Biol. 1968; 33(1):251-63. DOI: 10.1016/0022-2836(68)90292-1. View

19.

VAN Holde K, Sahasrabuddhe C, Shaw B . A model for particulate structure in chromatin. Nucleic Acids Res. 1974; 1(11):1579-86. PMC: 343437. DOI: 10.1093/nar/1.11.1579. View