Fractionation of Liver Chromatin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1971 Dec 1

PMID 5289239

Citations 24

Authors

K Marushige

J Bonner

Affiliations

Soon will be listed here.

Abstract

Rat liver chromatin has been fractionated into two fractions on the basis of precipitability in standard saline after mild treatment with DNase II. The major portion of liver chromatin contains little nonhistone protein and is enriched in histones, while a minor portion of such chromatin, with which RNA polymerase is associated, is highly enriched in proteins other than histone and impoverished in histones. Although the relative concentrations of the several histone species present in two chromatin fractions are identical, binding of histones to DNA in the minor fractions appears to be modified, presumably by the presence of proteins other than histones.

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