Urate Crystal-induced Chemotactic Factor: Isolation and Partial Characterization

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1976 Oct 1

PMID 965488

Citations 18

Authors

I Spilbert

A Gallacher

J M Mehta

B Mandell

Affiliations

Soon will be listed here.

Abstract

A factor with chemotactic properties for neutrophils and mononuclear cells was extracted from the lysosomal fraction of both human and rabbit neutrophils that had been allowed to phagocytose monosodium urate crystals. The chemotactic factor was found to be a glycoprotein with a mol wt of 8,400 daltons. The factor is heat labile and has chemotactic activity for human as well as rabbit cells. Preincubation of the cells with the urate induced chemotactic factor or with complement activated plasma prevents the cell from migrating chemotactically when challenged with either factor in the chemotactic chamber. The chemotactic factor induces release of lysosomal enzymes for cytochalasin B treated human neutrophils.

Citing Articles

Activation of human neutrophils by calcium carbonate polymorphs.

Burt H, Jackson J, Taylor D, Crowther R Dig Dis Sci. 1997; 42(6):1283-9.

PMID: 9201096 DOI: 10.1023/a:1018870511257.

Crystal-induced neutrophil activation. III. Inflammatory microcrystals induce a distinct pattern of tyrosine phosphorylation in human neutrophils.

Gaudry M, Roberge C, de Medicis R, Lussier A, Poubelle P, Naccache P J Clin Invest. 1993; 91(4):1649-55.

PMID: 8386191 PMC: 288143. DOI: 10.1172/JCI116373.

Crystal-induced neutrophil activation. IV. Specific inhibition of tyrosine phosphorylation by colchicine.

Roberge C, Gaudry M, de Medicis R, Lussier A, Poubelle P, Naccache P J Clin Invest. 1993; 92(4):1722-9.

PMID: 7691884 PMC: 288332. DOI: 10.1172/JCI116759.

Prolonged reduction in polymorphonuclear adhesion following oral colchicine.

Fordham J, Kirwan J, Cason J, Currey H Ann Rheum Dis. 1981; 40(6):605-8.

PMID: 7332382 PMC: 1000839. DOI: 10.1136/ard.40.6.605.

Demonstration of calcium-dependent chemotactic factor activatable esterase activity in human neutrophils: relationship with chemotaxis and chemotactic deactivation.

Mehta J, Mandell B, Kleyman T, Spilberg I Inflammation. 1981; 5(2):89-101.

PMID: 7275278 DOI: 10.1007/BF00914199.

References

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

COHN Z, Wiener E . THE PARTICULATE HYDROLASES OF MACROPHAGES. I. COMPARATIVE ENZYMOLOGY, ISOLATION, AND PROPERTIES. J Exp Med. 1963; 118:991-1008. PMC: 2137687. DOI: 10.1084/jem.118.6.991. View

BOYDEN S . The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962; 115:453-66. PMC: 2137509. DOI: 10.1084/jem.115.3.453. View

COHN Z, Hirsch J . The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. J Exp Med. 1960; 112:983-1004. PMC: 2137322. DOI: 10.1084/jem.112.6.983. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Becker E, Showell H, Henson P, Hsu L . The ability of chemotactic factors to induce lysosomal enzyme release. I. The characteristics of the release, the importance of surfaces and the relation of enzyme release to chemotactic responsiveness. J Immunol. 1974; 112(6):2047-54. View

Goetzl E, Austen K . A method for assessing the in vitro chemotactic response of neutrophils utilizing 51cr-labeled human leukocytes. Immunol Commun. 1972; 1(5):421-30. DOI: 10.3109/08820137209022954. View

Boyum A . Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968; 97:77-89. View

Zacharius R, Zell T, Morrison J, Woodlock J . Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969; 30(1):148-52. DOI: 10.1016/0003-2697(69)90383-2. View

10.

Gallin J, Durocher J, Kaplan A . Interaction of leukocyte chemotactic factors with the cell surface. I. Chemotactic factor-induced changes in human granulocyte surface charge. J Clin Invest. 1975; 55(5):967-74. PMC: 301842. DOI: 10.1172/JCI108026. View

11.

Goetzl E, Austen K . Active site chemotactic factors and the regulation of the human neutrophil chemotactic response. Antibiot Chemother (1971). 1974; 19:218-32. DOI: 10.1159/000395433. View

12.

Spilberg I, Mandell B, WOCHNER R . Studies on crystal-induced chemotactic factor. I. Requirement for protein synthesis and neutral protease activity. J Lab Clin Med. 1974; 83(1):56-63. View

13.

Zigmond S . A modified millipore filter method for assaying polymorphonuclear leukocyte locomotion and chemotaxis. Antibiot Chemother (1971). 1974; 19:126-45. DOI: 10.1159/000395428. View

14.

Stecher V . The chemotaxis of selected cell types to connective tissue degradation products. Ann N Y Acad Sci. 1975; 256:177-89. DOI: 10.1111/j.1749-6632.1975.tb36046.x. View

15.

Brot F, Glaser J, Roozen K, Sly W, Stahl P . In vitro correction of deficient human fibroblasts by beta-glucuronidase from different human sources. Biochem Biophys Res Commun. 1974; 57(1):1-8. DOI: 10.1016/s0006-291x(74)80349-9. View

16.

Gallin J, Clark R, Kimball H . Granulocyte chemotaxis: an improved in vitro assay employing 51 Cr-labeled granulocytes. J Immunol. 1973; 110(1):233-40. View

17.

Goldstein I, Hoffstein S, Weissmann G . Influence of divalent cations upon complement-mediated enzyme release from human polymorphonuclear leukocytes. J Immunol. 1975; 115(3):665-70. View

18.

Spilberg I, Gallacher A, Mendell B . Studies on crystal-induced chemotactic factor. II. Role of phagocytosis. J Lab Clin Med. 1975; 85(4):631-6. View

19.

Stahl P, Touster O . Beta-glucuronidase of rat liver lysosomes. Purification, properties, subunits. J Biol Chem. 1971; 246(17):5398-406. View

20.

Ward P, Newman L . A neutrophil chemotactic factor from human C'5. J Immunol. 1969; 102(1):93-9. View