Ceftazidime and Aztreonam Resistance in Providencia Stuartii: Characterization of a Natural TEM-derived Extended-spectrum Beta-lactamase, TEM-60

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1998 Jun 13

PMID 9624494

Citations 12

Authors

N Franceschini

M Perilli

B Segatore

D Setacci

G Amicosante

A Mazzariol

G Cornaglia

Affiliations

Soon will be listed here.

Abstract

A plasmid-encoded beta-lactamase produced from a clinical strain of Providencia stuartii has been purified and characterized. The gene coding for the beta-lactamase was cloned and sequenced. It appears to be a new natural TEM-derived enzyme, named TEM-60. Point mutations (Q39K, L51P, E104K, and R164S) are present with respect to the TEM-1 enzyme; the mutation L51P has never been previously reported, with the exception of the chromosomally encoded extended-spectrum beta-lactamase PER-1. Kinetic parameters relative to penicillins, cephalosporins, and monobactams other than mechanism-based inactivators were related to the in vitro susceptibility phenotype.

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