Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-producing Escherichia Coli Strains from Calves

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1998 Jun 10

PMID 9620426

Citations 66

Authors

S M Franck

B T Bosworth

H W Moon

Affiliations

Soon will be listed here.

Abstract

A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.

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