Enteropathogenic and Multidrug-Resistant -Carrying Isolates from Dogs and Cats

Overview

Journal Animals (Basel)

Date 2024 Sep 14

PMID 39272248

Authors

Catherine Biondo Feitosa

Gabriel Siqueira Dos Santos

Natalia Carrillo Gaeta

Gustavo da Silva Schiavi

Carla Gasparotto Chande Vasconcelos

Jonas Moraes Filho

Marcos Bryan Heinemann

Adriana Cortez

Affiliations

Soon will be listed here.

Abstract

Enteropathogenic (EPEC) are pathogens associated with gastrointestinal illnesses. Dogs and cats can harbor EPEC, and antimicrobial resistance may impair necessary treatments. This study characterized strains from dogs and cats, focusing on phylogroup classification, virulence factors, and antimicrobial resistance profiles. Ninety-seven isolates from fecal samples of 31 dogs and 3 cats were obtained from a private diagnostic laboratory in Botucatu, Brazil, from March to October 2021. The antimicrobial susceptibility was assessed using the disk diffusion method. Polymerase chain reaction (PCR) was employed to screen for and genes encoding virulence factors, as well as to classify the isolates into phylogroups. Twenty isolates were positive for intimin encoding gene and, consequently, these isolates were classified as EPEC (20.62%). Notably, 5.1% (5/97) of the isolates exhibited extended-spectrum β-lactamase (ESBL) production and 13.4% (13/97) were identified as multidrug-resistant bacteria. Phylogroups A and B2 were the most prevalent, comprising 29.9% (29/97) and 26.8% (26/97) of the bacterial isolates, respectively. This characterization highlights the prevalence of EPEC in domestic animals, emphasizing the potential risk they pose to public health and highlighting the urgency of responsible antimicrobial use in veterinary practices and the important role of laboratories in the surveillance of pathogenic multidrug-resistant bacteria.

References

Franck S, Bosworth B, Moon H . Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves. J Clin Microbiol. 1998; 36(6):1795-7. PMC: 104926. DOI: 10.1128/JCM.36.6.1795-1797.1998. View

Sato T, Yokota S, Okubo T, Usui M, Fujii N, Tamura Y . Phylogenetic association of fluoroquinolone and cephalosporin resistance of D-O1-ST648 Escherichia coli carrying blaCMY-2 from faecal samples of dogs in Japan. J Med Microbiol. 2013; 63(Pt 2):263-270. DOI: 10.1099/jmm.0.054676-0. View

Zogg A, Simmen S, Zurfluh K, Stephan R, Schmitt S, Nuesch-Inderbinen M . High Prevalence of Extended-Spectrum β-Lactamase Producing Enterobacteriaceae Among Clinical Isolates From Cats and Dogs Admitted to a Veterinary Hospital in Switzerland. Front Vet Sci. 2018; 5:62. PMC: 5890143. DOI: 10.3389/fvets.2018.00062. View

Lescat M, Clermont O, Woerther P, Glodt J, Dion S, Skurnik D . Commensal Escherichia coli strains in Guiana reveal a high genetic diversity with host-dependant population structure. Environ Microbiol Rep. 2013; 5(1):49-57. DOI: 10.1111/j.1758-2229.2012.00374.x. View

Clermont O, Christenson J, Denamur E, Gordon D . The Clermont Escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environ Microbiol Rep. 2013; 5(1):58-65. DOI: 10.1111/1758-2229.12019. View

Fan H, Kleven S, Jackwood M . Application of polymerase chain reaction with arbitrary primers to strain identification of Mycoplasma gallisepticum. Avian Dis. 1995; 39(4):729-35. View

Magiorakos A, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C . Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2011; 18(3):268-81. DOI: 10.1111/j.1469-0691.2011.03570.x. View

Kjaergaard A, Carr A, Gaunt M . Enteropathogenic Escherichia coli (EPEC) infection in association with acute gastroenteritis in 7 dogs from Saskatchewan. Can Vet J. 2016; 57(9):964-8. PMC: 4982568. View

Johnson T, Armstrong J, Johnston B, Merino-Velasco I, Jamborova I, Singer R . Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota. Zoonoses Public Health. 2022; 69(7):888-895. PMC: 9796152. DOI: 10.1111/zph.12985. View

10.

Paterson D, Bonomo R . Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 2005; 18(4):657-86. PMC: 1265908. DOI: 10.1128/CMR.18.4.657-686.2005. View

11.

Jin M, Osman M, Green B, Yang Y, Ahuja A, Lu Z . Evidence for the transmission of antimicrobial resistant bacteria between humans and companion animals: A scoping review. One Health. 2023; 17:100593. PMC: 10336692. DOI: 10.1016/j.onehlt.2023.100593. View

12.

Treier A, Stephan R, Stevens M, Cernela N, Nuesch-Inderbinen M . High Occurrence of Shiga Toxin-Producing in Raw Meat-Based Diets for Companion Animals-A Public Health Issue. Microorganisms. 2021; 9(8). PMC: 8398718. DOI: 10.3390/microorganisms9081556. View

13.

Sallem R, Gharsa H, Slama K, Rojo-Bezares B, Estepa V, Porres-Osante N . First detection of CTX-M-1, CMY-2, and QnrB19 resistance mechanisms in fecal Escherichia coli isolates from healthy pets in Tunisia. Vector Borne Zoonotic Dis. 2013; 13(2):98-102. DOI: 10.1089/vbz.2012.1047. View

14.

Gonzalez A, Cerqueira A . Shiga toxin-producing Escherichia coli in the animal reservoir and food in Brazil. J Appl Microbiol. 2019; 128(6):1568-1582. DOI: 10.1111/jam.14500. View

15.

Ghafourian S, Sadeghifard N, Soheili S, Sekawi Z . Extended Spectrum Beta-lactamases: Definition, Classification and Epidemiology. Curr Issues Mol Biol. 2014; 17:11-21. View

16.

Yun C, Moon B, Hwang M, Lee S, Ku B, Lee K . Characterization of the pathogenicity of extraintestinal pathogenic Escherichia coli isolates from pneumonia-infected lung samples of dogs and cats in South Korea. Sci Rep. 2023; 13(1):5575. PMC: 10076304. DOI: 10.1038/s41598-023-32287-z. View

17.

Clermont O, Bonacorsi S, Bingen E . Characterization of an anonymous molecular marker strongly linked to Escherichia coli strains causing neonatal meningitis. J Clin Microbiol. 2004; 42(4):1770-2. PMC: 387582. DOI: 10.1128/JCM.42.4.1770-1772.2004. View

18.

Bibbal D, Ruiz P, Sapountzis P, Mazuy-Cruchaudet C, Loukiadis E, Auvray F . Persistent Circulation of Enterohemorrhagic (EHEC) O157:H7 in Cattle Farms: Characterization of Enterohemorrhagic O157:H7 Strains and Fecal Microbial Communities of Bovine Shedders and Non-shedders. Front Vet Sci. 2022; 9:852475. PMC: 8994043. DOI: 10.3389/fvets.2022.852475. View

19.

Bonnet R, Dutour C, Sampaio J, Chanal C, Sirot D, Labia R . Novel cefotaximase (CTX-M-16) with increased catalytic efficiency due to substitution Asp-240-->Gly. Antimicrob Agents Chemother. 2001; 45(8):2269-75. PMC: 90641. DOI: 10.1128/AAC.45.8.2269-2275.2001. View

20.

Rodrigues J, Thomazini C, Lopes C, Dantas L . Concurrent infection in a dog and colonization in a child with a human enteropathogenic Escherichia coli clone. J Clin Microbiol. 2004; 42(3):1388-9. PMC: 356906. DOI: 10.1128/JCM.42.3.1388-1389.2004. View