Death-effector Filaments: Novel Cytoplasmic Structures That Recruit Caspases and Trigger Apoptosis

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1998 Jun 12

PMID 9606215

Citations 81

Authors

R M Siegel

D A Martin

L Zheng

S Y Ng

J Bertin

J Cohen

M J Lenardo

Affiliations

Soon will be listed here.

Abstract

The death-effector domain (DED) is a critical protein interaction domain that recruits caspases into complexes with members of the TNF-receptor superfamily. Apoptosis can also be induced by expressing certain DED-containing proteins without surface receptor cross-linking. Using Green Fluorescent Protein to examine DED-containing proteins in living cells, we show that these proteins cause apoptosis by forming novel cytoplasmic filaments that recruit and activate pro-caspase zymogens. Formation of these filaments, which we term death-effector filaments, was blocked by coexpression of viral antiapoptotic DED-containing proteins, but not by bcl-2 family proteins. Thus, formation of death-effector filaments allows a regulated intracellular assembly of apoptosis-signaling complexes that can initiate or amplify apoptotic stimuli independently of receptors at the plasma membrane.

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